| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,915,241 | A→G | E48G (GAG→GGG) | glyQ → | glycine tRNA synthetase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,915,241 | 0 | A | G | 100.0% | 74.4 / NA | 21 | E48G (GAG→GGG) | glyQ | glycine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (12/9); total (12/9) | |||||||||||
CATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACC > W3110S.gb/3915187‑3915296 | cATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTggg > 1:1484936/1‑61 (MQ=255)cATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTggg > 1:2625324/1‑61 (MQ=255) gTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATggc < 1:4640012/62‑1 (MQ=255) gTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATggc < 1:1193025/62‑1 (MQ=255) gTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATggc < 1:341843/62‑1 (MQ=255) gcgcGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGgct > 1:4274378/1‑62 (MQ=255) gAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTAt > 1:1308497/1‑62 (MQ=255) gAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTAt > 1:2451225/1‑62 (MQ=255) tctcACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCa < 1:3300921/62‑1 (MQ=255) ctcACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCa < 1:3433171/61‑1 (MQ=255) aTGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcg > 1:4258380/1‑62 (MQ=255) aTGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcg > 1:1528497/1‑62 (MQ=255) gTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccg < 1:431935/62‑1 (MQ=255) gTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccg < 1:480164/62‑1 (MQ=255) cTGCGCGGGCTGGGGCCAGAACCGATGGCGgctgct > 1:32397/1‑36 (MQ=255) cTGCGCGGGCTGGGGCCAGAACCGATGGCGgctgct > 1:4547241/1‑36 (MQ=255) cTGCGCGGGCTGGGGCCAGAACCGATGGCGgctgct > 1:55991/1‑36 (MQ=255) cTGCGCGGGCTGGGGCCAGAACCGATGGCGgctgct > 1:709574/1‑36 (MQ=255) cTGCGCGGGCTGGGGCCAGAACCGATGGCGgctgct > 1:805789/1‑36 (MQ=255) tGCGCGGGCTGGGGCCAGAACCGGTGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCccgacc < 1:875687/62‑1 (MQ=255) tGCGCGGGCTGGGGCCAGAACCGATGGCTGCTGCTTATGTTCAGCCTTCTCGTCGCccgacc < 1:2242394/62‑1 (MQ=255) | CATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACC > W3110S.gb/3915187‑3915296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |