Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	464,946	A→G	F531L (TTC→CTC)	ybaE ←	predicted transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	464,946	0	A	G	100.0%	271.0 / NA	72	F531L (TTC→CTC)	ybaE	predicted transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/31);  total (41/31)

CACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTA  >  W3110S.gb/464901‑465004                                              |                                                           cacCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGt                                             >  1:2997298/1‑61 (MQ=255) cacCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGt                                             >  1:4430356/1‑61 (MQ=255) cacCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGt                                             >  1:2936695/1‑61 (MQ=255) cacCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGt                                             >  1:4467177/1‑61 (MQ=255) cacCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGt                                             >  1:20224/1‑61 (MQ=255) cacCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGt                                             >  1:2820383/1‑61 (MQ=255) cacCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGt                                             >  1:2708381/1‑61 (MQ=255) cacCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGt                                             >  1:2722902/1‑61 (MQ=255)  acCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCg                                           <  1:1505529/62‑1 (MQ=255)  acCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCg                                           <  1:2376215/62‑1 (MQ=255)  acCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCg                                           <  1:3232997/62‑1 (MQ=255)   ccGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCg                                           <  1:1245928/61‑1 (MQ=255)   ccGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCg                                           <  1:2599478/61‑1 (MQ=255)   ccGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCg                                           <  1:3812271/61‑1 (MQ=255)   ccGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCg                                           <  1:4506865/61‑1 (MQ=255)     gTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCAt                                        >  1:1611217/1‑62 (MQ=255)      ttCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:2137715/62‑1 (MQ=255)      ttCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:3292983/62‑1 (MQ=255)      ttCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:2120908/62‑1 (MQ=255)               ggAGGGGCACTAATGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTAATTaa                              >  1:1366136/1‑62 (MQ=255)                           aTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:326070/41‑1 (MQ=255)                           aTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:3214375/41‑1 (MQ=255)                           aTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:339421/41‑1 (MQ=255)                           aTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:2291279/41‑1 (MQ=255)                           aTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:2211034/41‑1 (MQ=255)                           aTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:2189160/41‑1 (MQ=255)                           aTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:353423/41‑1 (MQ=255)                           aTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:3691608/41‑1 (MQ=255)                           aTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:1674190/41‑1 (MQ=255)                           aTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:904232/41‑1 (MQ=255)                           aTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:1299724/41‑1 (MQ=255)                           aTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAAc                  >  1:3697110/1‑62 (MQ=255)                           aTGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAAc                  >  1:2721632/1‑62 (MQ=255)                            tGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATc                                       <  1:344685/40‑1 (MQ=255)                            tGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTaaa                     >  1:2681508/1‑58 (MQ=255)                            tGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAAcc                 >  1:385803/1‑62 (MQ=255)                            tGCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAAcc                 >  1:3579802/1‑62 (MQ=255)                             gCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAAc                  <  1:4019176/60‑1 (MQ=255)                             gCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAAc                  <  1:2686141/60‑1 (MQ=255)                             gCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAAc                  <  1:4107914/60‑1 (MQ=255)                             gCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAAc                  <  1:2475615/60‑1 (MQ=255)                             gCGATAGTGATAGTTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAAc                  <  1:3495287/60‑1 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCAtt                                >  1:4096020/1‑41 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCAtt                                >  1:991962/1‑41 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCAtt                                >  1:4467591/1‑41 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCAtt                                >  1:4587952/1‑41 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCAtt                                >  1:748873/1‑41 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCAtt                                >  1:3668699/1‑41 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCAtt                                >  1:3546399/1‑41 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCAtt                                >  1:1275735/1‑41 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCAtt                                >  1:1382648/1‑41 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCAtt                                >  1:1752915/1‑41 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCAtt                                >  1:2336803/1‑41 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCAtt                                >  1:2647252/1‑41 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCAtt                                >  1:2931317/1‑41 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCAtt                                >  1:3127511/1‑41 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCt              >  1:2193577/1‑59 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCt              >  1:3549337/1‑59 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCt              >  1:3381005/1‑59 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCt              >  1:650161/1‑59 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCt              >  1:617088/1‑59 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCt              >  1:208426/1‑59 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCt              >  1:3975647/1‑59 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCt              >  1:2630910/1‑59 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCt              >  1:4345058/1‑59 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCt              >  1:2857520/1‑59 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCt              >  1:4036862/1‑59 (MQ=255)                                  agtgatagtTGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGATAAAAACCGCt              >  1:2369741/1‑59 (MQ=38)                                           tGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTa  <  1:3326860/62‑1 (MQ=255)                                           tGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTa  <  1:543459/62‑1 (MQ=255)                                           tGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTa  <  1:649005/62‑1 (MQ=255)                                           tGAGCAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTa  <  1:3765531/62‑1 (MQ=255)                                              |                                                           CACCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTA  >  W3110S.gb/464901‑465004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑