Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,996,085	C→T	A284V (GCC→GTC)	prlC →	oligopeptidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,996,085	0	C	T	100.0%	96.5 / NA	30	A284V (GCC→GTC)	prlC	oligopeptidase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/9);  total (21/9)

CGTCACGAACTGGCGCAACTGCTGGGCTTTGAAAACTACGCCTTTAAATCCCTTGCCACTAAAATGGCAGAAAACCCGCAGCAGGTGCTGGATTTCTTAACCGATCTGGCAAA  >  W3110S.gb/3996030‑3996142                                                        |                                                          cGTCACGAACTGGCGCAACTGCTGGGCTTTGAAAACTACGCCTTTAAATCCCTTGTCACTaa                                                     >  1:3217413/1‑62 (MQ=255) cGTCACGAACTGGCGCAACTGCTGGGCTTTGAAAACTACGCCTTTAAATCCCTTGTCACTaa                                                     >  1:275221/1‑62 (MQ=255)       gAACTGGCGCAACTGCTGGGCTTTGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATgg                                                <  1:3951423/61‑1 (MQ=255)       gAACTGGCGCAACTGCTGGGCTTTGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATgg                                                <  1:4292223/61‑1 (MQ=255)           tGGCGCAACTGCTGGGCTTTGAAAACTACGCCTTTAAATCCCTTGTCACTaaaa                                                   >  1:965098/1‑54 (MQ=255)           tGGCGCAACTGCTGGGCTTTGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGaa                                           >  1:3442653/1‑62 (MQ=255)           tGGCGCAACTGCTGGGCTTTGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGaa                                           >  1:3405177/1‑62 (MQ=255)           tGGCGCAACTGCTGGGCTTTGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGaa                                           >  1:109068/1‑62 (MQ=255)           tGGCGCAACTGCTGGGCTTTGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGaa                                           >  1:3996856/1‑62 (MQ=255)           tGGCGCAACTGCTGGGCTTTGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGaa                                           >  1:4199956/1‑62 (MQ=255)           tGGCGCAACTGCTGGGCTTTGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGaa                                           >  1:1610151/1‑62 (MQ=255)           tGGCGCAACTGCTGGGCTTTGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGaa                                           >  1:1311694/1‑62 (MQ=255)                           cTTTGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGAAAACCCGCAGCAGGTGc                           <  1:2085968/62‑1 (MQ=255)                           cTTTGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGAAAACCCGCAGCAGGTGc                           <  1:572943/62‑1 (MQ=255)                           cTTTGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGAAAACCCGCAGCAGGTGc                           <  1:1313857/62‑1 (MQ=255)                            tttGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGAAAACCCgcagca                                >  1:4168529/1‑56 (MQ=255)                            tttGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGAAAACCCgcagca                                >  1:699610/1‑56 (MQ=255)                            tttGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGAAAACCCgcagca                                >  1:3232523/1‑56 (MQ=255)                            tttGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGAAAACCCgcagca                                >  1:2344033/1‑56 (MQ=255)                            tttGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGAAAACCCgcagca                                >  1:2226199/1‑56 (MQ=255)                            tttGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATGGCAGAAAACCCgcagca                                >  1:1212345/1‑56 (MQ=255)                            tttGAAAACTACGCCTTTAAATCCCTTGTCACTAAAATAGCAGAAAACCCgcagca                                >  1:702247/1‑56 (MQ=255)                                           tttAAATCCCTTGTCACTAAAATGGCAGAAAACCCgcagca                                >  1:3626831/1‑41 (MQ=255)                                                 tCCCTTGTCACTAAAATGGCAGAAAACCCGCAGCAGGTGCTGGATTTCTTAACCGATCTgg      <  1:2503336/61‑1 (MQ=255)                                                 tCCCTTGTCACTAAAATGGCAGAAAACCCGCAGCAGGTGCTGGATTTCTTAACCGATCTgg      <  1:1755845/61‑1 (MQ=255)                                                 tCCCTTGTCACTAAAATGGCAGAAAACCCGCAGCAGGTGCTGGATTTCTTAACCGATCTgg      <  1:1304378/61‑1 (MQ=255)                                                 tCCCTTGTCACTAAAATGGCAGAAAACCCGCAGCAGGTGCTGGATTTCTTAACCGATCTgg      <  1:905431/61‑1 (MQ=255)                                                    cTTGTCACTAAAATGGCAGAAAACCCGCAGCAGGTGCTGGATTTCTTAACCGATCTGGCaaa  >  1:3498995/1‑62 (MQ=255)                                                    cTTGTCACTAAAATGGCAGAAAACCCGCAGCAGGTGCTGGATTTCTTAACCGATCTGGCaaa  >  1:1876308/1‑62 (MQ=255)                                                    cTTGTCACTAAAATGGCAGAAAACCCGCAGCAGGTGCTGGATTTCTTAACCGATCTGGCaaa  >  1:1329077/1‑62 (MQ=255)                                                        |                                                          CGTCACGAACTGGCGCAACTGCTGGGCTTTGAAAACTACGCCTTTAAATCCCTTGCCACTAAAATGGCAGAAAACCCGCAGCAGGTGCTGGATTTCTTAACCGATCTGGCAAA  >  W3110S.gb/3996030‑3996142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑