Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,012,376	G→A	A500T (GCC→ACC)	rbbA →	fused ribosome‑associated ATPases

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,012,376	0	G	A	100.0%	67.2 / NA	23	A500T (GCC→ACC)	rbbA	fused ribosome‑associated ATPases
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/15);  total (8/15)

TGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGG  >  W3110S.gb/4012317‑4012426                                                            |                                                   tGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAacc                                                  >  1:1301756/1‑62 (MQ=255) tGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAacc                                                  >  1:65006/1‑62 (MQ=255) tGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAacc                                                  >  1:1750678/1‑62 (MQ=255) tGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAacc                                                  >  1:4444816/1‑62 (MQ=255) tGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAacc                                                  >  1:2046957/1‑62 (MQ=255) tGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAacc                                                  >  1:3980433/1‑62 (MQ=255) tGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAacc                                                  >  1:3326121/1‑62 (MQ=255) tGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAacc                                                  >  1:3260437/1‑62 (MQ=255) tGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAacc                                                  >  1:28374/1‑62 (MQ=255)      gCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACACGGAACCGCCAg                                             >  1:4280018/1‑62 (MQ=255)            aaaGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGa                                       <  1:987298/62‑1 (MQ=255)            aaaGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGa                                       <  1:4486760/62‑1 (MQ=255)            aaaGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGa                                       <  1:2393961/62‑1 (MQ=255)               gTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAaga                                    <  1:3171687/62‑1 (MQ=255)                         gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAagag                                   >  1:1556452/1‑53 (MQ=255)                         gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATc                          >  1:3404794/1‑62 (MQ=255)                         gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATc                          >  1:1786555/1‑62 (MQ=255)                               cacCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAaga                                    <  1:2747057/46‑1 (MQ=255)                               cacCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAaga                                    <  1:342534/46‑1 (MQ=255)                               cacCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAaga                                    <  1:3588550/46‑1 (MQ=255)                               cacCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAaga                                    <  1:228690/46‑1 (MQ=255)                               cacCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAaga                                    <  1:20404/46‑1 (MQ=255)                                acCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAaga                                    <  1:758905/45‑1 (MQ=255)                                    cAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGc                >  1:4205360/1‑61 (MQ=255)                                     aGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAg              <  1:1415383/62‑1 (MQ=255)                                     aGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAg              <  1:913976/62‑1 (MQ=255)                                     aGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAg              <  1:446529/62‑1 (MQ=255)                                     aGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAg              <  1:4218609/62‑1 (MQ=255)                                     aGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAg              <  1:3134996/62‑1 (MQ=255)                                      ggAACTGGTTGAGAAACGCGGTACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCa               >  1:2188015/1‑60 (MQ=255)                                      ggAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCg                         >  1:3489179/1‑50 (MQ=255)                                                   aaaCGCGGAACCTCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcaggg  >  1:2542128/1‑60 (MQ=255)                                                            |                                                   TGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGG  >  W3110S.gb/4012317‑4012426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑