Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,036,723	G→A	A184T (GCT→ACT)	ftsY →	fused Signal Recognition Particle (SRP) receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,036,723	0	G	A	100.0%	77.6 / NA	25	A184T (GCT→ACT)	ftsY	fused Signal Recognition Particle (SRP) receptor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/16);  total (9/16)

GTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCT  >  W3110S.gb/4036681‑4036781                                           |                                                           gtggtTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAAcc                                         >  1:1696112/1‑62 (MQ=255)     ttCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGAc                                      >  1:2144990/1‑61 (MQ=255)         tccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCaaa                                  <  1:4319798/61‑1 (MQ=255)         tccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCaaa                                  <  1:415978/61‑1 (MQ=255)         tccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCaaa                                  <  1:3886433/61‑1 (MQ=255)         tccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCaa                                   >  1:4575928/1‑60 (MQ=255)         tccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCaa                                   >  1:128452/1‑60 (MQ=255)         tccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCaa                                   >  1:2155789/1‑60 (MQ=255)                   gaagaGCAGCCGGTGGAAGAAATCACTCAGGAGCTGGAAAAACCGACCAAAGAAGGttttt                        <  1:365526/61‑1 (MQ=39)                   gaagaGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGttttt                        <  1:3002434/61‑1 (MQ=255)                   gaagaGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGttttt                        <  1:379614/61‑1 (MQ=255)                   gaagaGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGttttt                        <  1:3560152/61‑1 (MQ=255)                   gaagaGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGttttt                        <  1:1627619/61‑1 (MQ=255)                   gaagaGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGttttt                        <  1:1518712/61‑1 (MQ=255)                      gagCAGCCGGTGGAAGAAATCACTCAGGAGCTGGAAAAACCGACCaaa                                  <  1:2636783/48‑1 (MQ=37)                       agcagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcg                   <  1:3156905/62‑1 (MQ=255)                       agcagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcg                   <  1:1106630/62‑1 (MQ=255)                        gcagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcgc                  <  1:1088390/62‑1 (MQ=255)                        gcagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcgc                  <  1:3174989/62‑1 (MQ=255)                        gcagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcgc                  <  1:4089695/62‑1 (MQ=255)                         cagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcgcg                 >  1:3723340/1‑62 (MQ=255)                         cagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcgcg                 >  1:1366324/1‑62 (MQ=255)                         cagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcgcg                 >  1:1297718/1‑62 (MQ=255)                         cagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcgc                  <  1:1911668/61‑1 (MQ=255)                                        aTCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCt  >  1:3656827/1‑62 (MQ=255)                                           |                                                           GTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCT  >  W3110S.gb/4036681‑4036781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑