Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,064,477	A→G	E374E (GAA→GAG)	gntU →	gluconate transporter, low affinity GNT 1 system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,064,477	0	A	G	100.0%	143.3 / NA	39	E374E (GAA→GAG)	gntU	gluconate transporter, low affinity GNT 1 system
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/18);  total (21/18)

CGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAACAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTATCGCTGGTGGTTC  >  W3110S.gb/4064432‑4064536                                              |                                                            cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg                                             <  1:4168183/62‑1 (MQ=255) cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg                                             <  1:2908937/62‑1 (MQ=255) cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg                                             <  1:1546833/62‑1 (MQ=255) cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg                                             <  1:3201746/62‑1 (MQ=255) cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg                                             <  1:1614985/62‑1 (MQ=255) cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg                                             <  1:2672918/62‑1 (MQ=255) cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg                                             <  1:1769418/62‑1 (MQ=255) cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg                                             <  1:2124922/62‑1 (MQ=255) cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg                                             <  1:239965/62‑1 (MQ=255)                   ggTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATggcggc                             >  1:3158092/1‑60 (MQ=255)                   ggTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGgcgc                           >  1:2174476/1‑62 (MQ=255)                   ggTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGgcgc                           >  1:2423998/1‑62 (MQ=255)                   ggTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGgcgc                           >  1:3360209/1‑62 (MQ=255)                   ggTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGgcgc                           >  1:1722121/1‑62 (MQ=255)                   ggTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGgcgc                           >  1:1581616/1‑62 (MQ=255)                        gACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCg                      >  1:3317852/1‑62 (MQ=255)                             gTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 >  1:3621327/1‑62 (MQ=255)                             gTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 >  1:3599537/1‑62 (MQ=255)                             gTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 >  1:3594279/1‑62 (MQ=255)                             gTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 >  1:4118301/1‑62 (MQ=255)                             gTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 >  1:1210412/1‑62 (MQ=255)                             gTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 >  1:2308827/1‑62 (MQ=255)                             gTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 >  1:1017852/1‑62 (MQ=255)                              tGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGt                >  1:2394148/1‑62 (MQ=255)                               gATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 <  1:305742/60‑1 (MQ=255)                               gATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 <  1:2471929/60‑1 (MQ=255)                               gATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 <  1:2450749/60‑1 (MQ=255)                               gATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 <  1:3893525/60‑1 (MQ=255)                               gATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 <  1:4214082/60‑1 (MQ=255)                               gATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 <  1:4138785/60‑1 (MQ=255)                                aTGCCGGTTATTGAGCGACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTAt              >  1:306494/1‑62 (MQ=255)                                aTGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 <  1:2657948/59‑1 (MQ=255)                                aTGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg                 <  1:399174/59‑1 (MQ=255)                                aTGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTAt              >  1:984788/1‑62 (MQ=255)                                aTGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGAAt              >  1:746012/1‑62 (MQ=255)                                          ttGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTATCGCTggtggt    >  1:1410817/1‑62 (MQ=255)                                          ttGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTATCGCTggtggt    >  1:3985472/1‑62 (MQ=255)                                          ttGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTATCGCTggtggt    >  1:290662/1‑62 (MQ=255)                                           tGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCG‑‑GTCGATTTGTATCGCTGGTGGTTc  <  1:904949/61‑1 (MQ=255)                                              |                                                            CGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAACAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTATCGCTGGTGGTTC  >  W3110S.gb/4064432‑4064536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑