Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,083,312	G→A	G325E (GGG→GAG)	rtcB →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,083,312	0	G	A	100.0%	114.9 / NA	35	G325E (GGG→GAG)	rtcB	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/28);  total (7/28)

GCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCG  >  W3110S.gb/4083254‑4083356                                                           |                                             gCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGc                                            >  1:3502433/1‑61 (MQ=255) gCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCt                                           >  1:1372342/1‑62 (MQ=255) gCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCt                                           >  1:228165/1‑62 (MQ=255) gCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCt                                           >  1:1985553/1‑62 (MQ=255)        aaTATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAAt                                    <  1:3381468/62‑1 (MQ=255)        aaTATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAAt                                    <  1:3398343/62‑1 (MQ=255)        aaTATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAAt                                    <  1:1959581/62‑1 (MQ=255)        aaTATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAAt                                    <  1:726407/62‑1 (MQ=255)        aaTATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAAt                                    <  1:1470469/62‑1 (MQ=255)        aaTATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAAt                                    <  1:1441098/62‑1 (MQ=255)        aaTATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAAt                                    <  1:1425721/62‑1 (MQ=255)            tGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTggg                                        <  1:1868607/54‑1 (MQ=255)            tGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTggg                                        <  1:2035148/54‑1 (MQ=255)            tGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTggg                                        <  1:2076520/54‑1 (MQ=255)            tGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTggg                                        <  1:3740643/54‑1 (MQ=255)             ggAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTggg                                        <  1:1708083/53‑1 (MQ=255)                   attCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTg                                          <  1:625425/45‑1 (MQ=255)                   attCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTg                                          <  1:3725737/45‑1 (MQ=255)                   attCCCGGTTCGATGGGAGCAAAAAACTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGtt                         >  1:2559488/1‑62 (MQ=255)                          gTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCg                  >  1:170774/1‑62 (MQ=255)                            tCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGGGTCGTTCTGTTCGTg                <  1:1044152/62‑1 (MQ=255)                            tCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTg                <  1:2353200/62‑1 (MQ=255)                            tCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTg                <  1:4368881/62‑1 (MQ=255)                            tCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTg                <  1:950187/62‑1 (MQ=255)                            tCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTg                <  1:964244/62‑1 (MQ=255)                                         aaaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGcc   >  1:3902024/1‑62 (MQ=255)                                          aaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCcg  <  1:3217653/62‑1 (MQ=255)                                          aaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCcg  <  1:2547889/62‑1 (MQ=255)                                          aaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCcg  <  1:3506318/62‑1 (MQ=255)                                          aaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCcg  <  1:3582001/62‑1 (MQ=255)                                          aaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCcg  <  1:2134343/62‑1 (MQ=255)                                          aaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCcg  <  1:4072439/62‑1 (MQ=255)                                          aaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCcg  <  1:60041/62‑1 (MQ=255)                                          aaGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCcg  <  1:1540307/62‑1 (MQ=255)                                               ttATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTg                <  1:2999283/43‑1 (MQ=255)                                                           |                                             GCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCG  >  W3110S.gb/4083254‑4083356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑