BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,123,875	G→A	R255H (CGC→CAC)	damX →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,123,875	0	G	A	100.0%	210.1 / NA	70	R255H (CGC→CAC)	damX	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (54/16); total (54/16)

CATCGCGTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCGCCCAGCTCGTCAGCAGGCGGTGATTGAACCGAAAAA  >  W3110S.gb/4123824‑4123911

cATCGCGTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTc                              >  1:838746/1‑60 (MQ=255)

cATCGCGTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTc                              >  1:630599/1‑60 (MQ=255)

cATCGCGTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTc                              >  1:628392/1‑60 (MQ=255)

cATCGCGTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTc                              >  1:538316/1‑60 (MQ=255)

    gcgTGACACGGCGAAAACGCAAACCTCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc                        <  1:1828958/62‑1 (MQ=255)

    gcgTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc                        <  1:3150175/62‑1 (MQ=255)

    gcgTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc                        <  1:4040272/62‑1 (MQ=255)

    gcgTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc                        <  1:4135814/62‑1 (MQ=255)

    gcgTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc                        <  1:1222184/62‑1 (MQ=255)

    gcgTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc                        <  1:1827004/62‑1 (MQ=255)

    gcgTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc                        <  1:1395268/62‑1 (MQ=255)

    gcgTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc                        <  1:1465064/62‑1 (MQ=255)

      gTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagca                       <  1:1728329/61‑1 (MQ=255)

      gTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagca                       <  1:285379/61‑1 (MQ=255)

      gTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagca                       <  1:4010600/61‑1 (MQ=255)

      gTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagca                       <  1:213517/61‑1 (MQ=255)

      gTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagca                       <  1:1373443/61‑1 (MQ=255)

      gTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagca                       <  1:1007599/61‑1 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagcag                      >  1:4309938/1‑61 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag                         >  1:2097508/1‑58 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag                         >  1:1012247/1‑58 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag                         >  1:2917999/1‑58 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag                         >  1:2964097/1‑58 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag                         >  1:1101522/1‑58 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag                         >  1:2446956/1‑58 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag                         >  1:31851/1‑58 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag                         >  1:323264/1‑58 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag                         >  1:3568104/1‑58 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag                         >  1:640897/1‑58 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag                         >  1:1799084/1‑58 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag                         >  1:4518108/1‑58 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag                         >  1:4614030/1‑58 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag                         >  1:4619011/1‑58 (MQ=255)

       tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTca                          >  1:708170/1‑57 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:869423/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:463588/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:808305/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:899747/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:2683104/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:1124387/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:1188855/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:1505290/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:1512329/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:1716272/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:1784837/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:1843134/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:2089508/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:2369644/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:2803468/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:2604731/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:2554073/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg                                 >  1:2456996/1‑46 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt                 >  1:3591716/1‑62 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt                 >  1:1211692/1‑62 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt                 >  1:1383271/1‑62 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt                 >  1:1431810/1‑62 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt                 >  1:1572870/1‑62 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt                 >  1:1772751/1‑62 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt                 >  1:3186917/1‑62 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt                 >  1:4309162/1‑62 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt                 >  1:2433048/1‑62 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt                 >  1:3258464/1‑62 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt                 >  1:231941/1‑62 (MQ=255)

           acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt                 >  1:3482282/1‑62 (MQ=255)

                     cGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGTGATTGGACCg       <  1:2446568/62‑1 (MQ=255)

                          aCCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGTGATTGAACCGaaaaa  >  1:555794/1‑62 (MQ=255)

                          aCCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGTGATTGAACCGaaaaa  >  1:1142890/1‑62 (MQ=255)

                          aCCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGTGATTGAACCGaaaaa  >  1:1117513/1‑62 (MQ=255)

                          aCCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGTGATTGAACCGaaaaa  >  1:1115385/1‑62 (MQ=255)

                               tGAACGTCCGTCCACTACGCACCCAGCTCGTcagc                        <  1:681137/35‑1 (MQ=255)

CATCGCGTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCGCCCAGCTCGTCAGCAGGCGGTGATTGAACCGAAAAA  >  W3110S.gb/4123824‑4123911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`