| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 478,343 | C→T | A45T (GCT→ACT) | ylaC ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 478,343 | 0 | C | T | 100.0% | 19.1 / NA | 8 | A45T (GCT→ACT) | ylaC | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (2/6); total (2/6) | |||||||||||
GGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGAT > W3110S.gb/478308‑478382 | ggACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGcccc < 1:2962212/61‑1 (MQ=255)ggACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGcccc < 1:4399032/61‑1 (MQ=255) aCATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTa < 1:360322/62‑1 (MQ=255) cACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGa < 1:4697434/62‑1 (MQ=255) cACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGa < 1:698749/62‑1 (MQ=255) ccGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGAt > 1:3704145/1‑61 (MQ=255) cAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTa < 1:2589618/52‑1 (MQ=255) aaGTAACGTACATACCGATAAACAGCCCCGGATGTTTa > 1:129491/1‑38 (MQ=255) | GGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGAT > W3110S.gb/478308‑478382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |