BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,328,660	C→T	V125I (GTC→ATC)	phnD ←	phosphonate/organophosphate ester transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,328,660	0	C	T	100.0%	102.8 / NA	33	V125I (GTC→ATC)	phnD	phosphonate/organophosphate ester transporter subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (5/28); total (5/28)

TCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGG  >  W3110S.gb/4328605‑4328709

tCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCaa                                             <  1:3565535/62‑1 (MQ=255)

tCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCaa                                             <  1:3116072/62‑1 (MQ=255)

tCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCaa                                             <  1:2117102/62‑1 (MQ=255)

tCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCaa                                             <  1:1020600/62‑1 (MQ=255)

tCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCaa                                             <  1:4563041/62‑1 (MQ=255)

   ttCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAacac                                          >  1:140957/1‑62 (MQ=255)

   ttCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAacac                                          >  1:3555050/1‑62 (MQ=255)

      cGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGc                                        <  1:2642400/61‑1 (MQ=255)

                 cagATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCg                            >  1:1960619/1‑62 (MQ=255)

                 cagATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCg                            >  1:1437853/1‑62 (MQ=255)

                  agATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCgg                           <  1:1592734/62‑1 (MQ=255)

                  agATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCgg                           <  1:900244/62‑1 (MQ=255)

                            ggTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGatccatc                  <  1:1684077/61‑1 (MQ=255)

                            ggTTGTTGATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGatccatc                  <  1:337643/61‑1 (MQ=255)

                             gttgttGATCGGACTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATccg                >  1:2892932/1‑62 (MQ=255)

                                         aCTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCt    <  1:57085/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:3846553/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:3589866/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:2069840/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:3401218/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:55681/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:3137270/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:2218606/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:2023200/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:1738964/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:1566609/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:1462585/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:139562/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:1202039/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:112380/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTg   <  1:1077771/62‑1 (MQ=255)

                                          cTGTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATACATCCGCCGCGACCGTCTg   <  1:196648/62‑1 (MQ=255)

                                            gTCTTTGTTGATGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCTACCGTCTgg  <  1:1991193/61‑1 (MQ=255)

TCTTTCCGCTTCGCCAGCAGATCGTTCAGGTTGTTGATCGGACTGTCTTTGTTGACGATCAACACGCTCCAGTAACCCGGCGATCCATCCGCCGCGACCGTCTGG  >  W3110S.gb/4328605‑4328709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`