Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,392,090	G→T	L655M (CTG→ATG)	yjeP ←	predicted mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,392,090	0	G	T	100.0%	89.6 / NA	28	L655M (CTG→ATG)	yjeP	predicted mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/4);  total (24/4)

ACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATG  >  W3110S.gb/4392039‑4392145                                                    |                                                        acaGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCg                                               >  1:1903306/1‑62 (MQ=255)              ggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGc                                  <  1:1055582/62‑1 (MQ=255)              ggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGc                                  <  1:3111707/62‑1 (MQ=255)              ggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGc                                  <  1:2953191/62‑1 (MQ=255)              ggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTAAGGc                                  <  1:2477673/62‑1 (MQ=255)                tAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTc                                      >  1:1513469/1‑56 (MQ=255)                tAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTg                                >  1:112276/1‑62 (MQ=255)                tAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTg                                >  1:164469/1‑62 (MQ=255)                tAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTg                                >  1:2948765/1‑62 (MQ=255)                        tCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTg                                >  1:2669513/1‑54 (MQ=255)                        tCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac                        >  1:3786665/1‑62 (MQ=255)                        tCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac                        >  1:4073541/1‑62 (MQ=255)                        tCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac                        >  1:4121585/1‑62 (MQ=255)                        tCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac                        >  1:2511510/1‑62 (MQ=255)                        tCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac                        >  1:473827/1‑62 (MQ=255)                            cgcTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGaccacc                    >  1:4418279/1‑62 (MQ=255)                            cgcTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGaccacc                    >  1:3228103/1‑62 (MQ=255)                            cgcTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGaccacc                    >  1:3356400/1‑62 (MQ=255)                            cgcTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGaccacc                    >  1:335557/1‑62 (MQ=255)                            cgcTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGaccacc                    >  1:2766805/1‑62 (MQ=255)                            cgcTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGaccacc                    >  1:1196517/1‑62 (MQ=255)                            cgcTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac                        >  1:3846720/1‑58 (MQ=255)                                        tgttgAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCAcc         >  1:2468899/1‑61 (MQ=255)                                             agaTACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac                        >  1:3742498/1‑41 (MQ=255)                                             agaTACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac                        >  1:4036562/1‑41 (MQ=255)                                             agaTACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac                        >  1:927863/1‑41 (MQ=255)                                             agaTACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGac                        >  1:92877/1‑41 (MQ=255)                                               aTACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATg  >  1:3595232/1‑61 (MQ=255)                                                    |                                                        ACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATG  >  W3110S.gb/4392039‑4392145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑