Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,443,410	G→A	L179L (CTG→TTG)	ytfJ ←	predicted transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,443,410	0	G	A	100.0%	29.5 / NA	11	L179L (CTG→TTG)	ytfJ	predicted transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/4);  total (7/4)

TAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTG  >  W3110S.gb/4443351‑4443425                                                            |                tAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTgcaaca               <  1:4210243/62‑1 (MQ=255)   ggggAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAgg             <  1:3376029/62‑1 (MQ=255)   ggggAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAgg             <  1:3945982/62‑1 (MQ=255)   ggggAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAgg             <  1:741159/62‑1 (MQ=255)           ttattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCa      <  1:2127083/61‑1 (MQ=255)              tttttGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACTtg  >  1:1330703/1‑62 (MQ=255)              tttttGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACTtg  >  1:1420473/1‑62 (MQ=255)               ttttGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAgg             >  1:1812949/1‑50 (MQ=255)               ttttGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAgg             >  1:3329197/1‑50 (MQ=255)               ttttGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAgg             >  1:3370154/1‑50 (MQ=255)               ttttGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAgg             >  1:3375417/1‑50 (MQ=255)               ttttGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAgg             >  1:599108/1‑50 (MQ=255)                                                            |                TAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTG  >  W3110S.gb/4443351‑4443425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑