Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,477,260	G→A	intergenic (‑45/+236)	pyrL ← / ← yjgH	pyrBI operon leader peptide/predicted mRNA endoribonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,477,260	0	G	A	100.0%	128.2 / NA	41	intergenic (‑45/+236)	pyrL/yjgH	pyrBI operon leader peptide/predicted mRNA endoribonuclease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/11);  total (30/11)

ACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATT  >  W3110S.gb/4477202‑4477313                                                           |                                                      acacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacgg                                                    >  1:4300537/1‑62 (MQ=255) acacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacgg                                                    >  1:1439625/1‑62 (MQ=255)  cacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacggc                                                   <  1:2539889/62‑1 (MQ=255)  cacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacggc                                                   <  1:3352463/62‑1 (MQ=255)  cacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacggc                                                   <  1:1418474/62‑1 (MQ=255)   acacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacggc                                                   <  1:3862643/61‑1 (MQ=255)     acTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCa                                                >  1:1632227/1‑62 (MQ=255)     acTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCa                                                >  1:1730308/1‑62 (MQ=255)     acTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATAGTCAGAAACGGCGCa                                                >  1:3576478/1‑62 (MQ=255)     acTGAACCATACATCCTCCCGGCAAATTGTCCGGCATGATACTCATCGTCAGAAACGGCGCa                                                >  1:1709929/1‑62 (MQ=255)           ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt                                           <  1:380402/61‑1 (MQ=255)           ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt                                           <  1:4048859/61‑1 (MQ=255)           ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt                                           <  1:4227610/61‑1 (MQ=255)           ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt                                           <  1:1002447/61‑1 (MQ=255)           ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt                                           <  1:260900/61‑1 (MQ=255)           ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt                                           <  1:2594406/61‑1 (MQ=255)           ccATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt                                           <  1:882444/61‑1 (MQ=255)                cATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacgg                                                    <  1:1784834/47‑1 (MQ=255)                       ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCa                                                >  1:342273/1‑44 (MQ=255)                       ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAatat                               >  1:1749917/1‑61 (MQ=255)                       ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAatat                               >  1:2223873/1‑61 (MQ=255)                       ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATAtt                              >  1:3377954/1‑62 (MQ=255)                       ccGGCAAATTGTCCAGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATAtt                              >  1:759263/1‑62 (MQ=255)                           cAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAttt                                         >  1:1326621/1‑47 (MQ=255)                           cAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAttt                                         >  1:1148037/1‑47 (MQ=255)                           cAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAttt                                         >  1:123725/1‑47 (MQ=255)                           cAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAttt                                         >  1:3929710/1‑47 (MQ=255)                           cAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAttt                                         >  1:338025/1‑47 (MQ=255)                           cAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAttt                                         >  1:2114392/1‑47 (MQ=255)                                      gCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACg               >  1:38113/1‑62 (MQ=255)                                       cATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCaa                  >  1:3765879/1‑58 (MQ=255)                                             aCTCAACGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAt         >  1:294931/1‑61 (MQ=255)                                              cTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAtt        <  1:3457516/61‑1 (MQ=255)                                              cTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAtt        <  1:521526/61‑1 (MQ=255)                                              cTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAtt        <  1:1176839/61‑1 (MQ=255)                                              cTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTCTGTCAGCGCAAACGGTTTAtt        <  1:1198399/61‑1 (MQ=255)                                                  tCGTCAGAAACGGCGCAAGCATTTGATGCAATAtt                              >  1:4141816/1‑35 (MQ=255)                                                  tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGttt           >  1:2289711/1‑54 (MQ=255)                                                  tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGttt           >  1:774417/1‑54 (MQ=255)                                                  tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGttt           >  1:71302/1‑54 (MQ=255)                                                  tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGttt           >  1:3718456/1‑54 (MQ=255)                                                  tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGttt           >  1:1494529/1‑54 (MQ=255)                                                  tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGaa    >  1:2839436/1‑61 (MQ=255)                                                  tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGaa    >  1:1532404/1‑61 (MQ=255)                                                    gTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAAtt  >  1:4135944/1‑61 (MQ=255)                                                    gTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAAtt  >  1:617910/1‑61 (MQ=255)                                                    gTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAAtt  >  1:1300599/1‑61 (MQ=255)                                                    gTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAAtt  >  1:729724/1‑61 (MQ=255)                                                           |                                                      ACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATT  >  W3110S.gb/4477202‑4477313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑