Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,561,090	G→A	intergenic (‑88/‑585)	yjiC ← / → yjiD	hypothetical protein/DNA replication/recombination/repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,561,090	0	G	A	100.0%	150.5 / NA	46	intergenic (‑88/‑585)	yjiC/yjiD	hypothetical protein/DNA replication/recombination/repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/22);  total (24/22)

GATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAAC  >  W3110S.gb/4561031‑4561146                                                            |                                                         gATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcataca                                                        >  1:3619257/1‑62 (MQ=255)       tAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACGTGttt                                                   <  1:4232030/61‑1 (MQ=255)       tAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGttt                                                   <  1:3400437/61‑1 (MQ=255)       tAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGttt                                                   <  1:3025978/61‑1 (MQ=255)       tAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGttt                                                   <  1:3556502/61‑1 (MQ=255)       tAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGttt                                                   <  1:1443418/61‑1 (MQ=255)       tAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGttt                                                   <  1:3573906/61‑1 (MQ=255)       tAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGttt                                                   <  1:388396/61‑1 (MQ=255)               tAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAg                                          <  1:1268221/62‑1 (MQ=255)               tAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAg                                          <  1:1357799/62‑1 (MQ=255)               tAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAg                                          <  1:2901744/62‑1 (MQ=255)               tAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAg                                          <  1:411569/62‑1 (MQ=255)                   tAAATTTTTCTTTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGat                                      >  1:702096/1‑62 (MQ=255)                   tAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGat                                      >  1:783867/1‑62 (MQ=255)                   tAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGat                                      >  1:2900111/1‑62 (MQ=255)                   tAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGat                                      >  1:783863/1‑62 (MQ=255)                     aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGacatacat                                                       <  1:4194997/43‑1 (MQ=255)                     aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGacatacat                                                       <  1:432909/43‑1 (MQ=255)                     aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGacatacat                                                       <  1:2459255/43‑1 (MQ=255)                     aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGacatacat                                                       <  1:3928103/43‑1 (MQ=255)                     aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGacatacat                                                       <  1:2703014/43‑1 (MQ=255)                     aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGacatacat                                                       <  1:1900336/43‑1 (MQ=255)                     aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGacatacat                                                       <  1:2273377/43‑1 (MQ=255)                     aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGacatacat                                                       <  1:2752530/43‑1 (MQ=255)                     aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGacatacat                                                       <  1:182897/43‑1 (MQ=255)                     aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGacatacat                                                       <  1:1781875/43‑1 (MQ=255)                     aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGacatacat                                                       <  1:1619156/43‑1 (MQ=255)                     aaTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGacatacat                                                       <  1:1283205/43‑1 (MQ=255)                         tttCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCtg                                                <  1:3837208/46‑1 (MQ=255)                         tttCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCtg                                                <  1:2410903/46‑1 (MQ=255)                          ttCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAAc                                >  1:4502802/1‑61 (MQ=255)                          ttCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAAc                                >  1:1139252/1‑61 (MQ=255)                          ttCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAAc                                >  1:2802162/1‑61 (MQ=255)                            cTGTAGTAAAAAGAGAAGTAAACAAATGAcataca                                                        >  1:3281070/1‑35 (MQ=255)                            cTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCtg                                                >  1:2354217/1‑43 (MQ=255)                            cTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCtg                                                >  1:161316/1‑43 (MQ=255)                            cTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCtg                                                >  1:3200264/1‑43 (MQ=255)                            cTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCtg                                                >  1:2958540/1‑43 (MQ=255)                            cTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCtg                                                >  1:2881766/1‑43 (MQ=255)                            cTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCt                                                 >  1:271386/1‑42 (MQ=255)                             tGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAActct                             <  1:2714428/61‑1 (MQ=255)                             tGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAActct                             <  1:3890974/61‑1 (MQ=255)                             tGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAActct                             <  1:1191635/61‑1 (MQ=255)                             tGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAActct                             <  1:245120/61‑1 (MQ=255)                             tGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAActct                             <  1:239335/61‑1 (MQ=255)                             tGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAActct                             <  1:4436997/61‑1 (MQ=255)                                agtaAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGt                                         >  1:2151845/1‑46 (MQ=255)                                agtaAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGt                                         >  1:559381/1‑46 (MQ=255)                                agtaAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGt                                         >  1:4705940/1‑46 (MQ=255)                                   aaaaaGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGt                      <  1:1143876/62‑1 (MQ=255)                                   aaaaaGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGt                      <  1:936088/62‑1 (MQ=255)                                   aaaaaGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGt                      <  1:945792/62‑1 (MQ=255)                                           aaGTAAACAAATGACATACATGTTTCTGTTGTGGTGATATCAACTCTACACGGTGATATTaa              >  1:3097374/1‑62 (MQ=255)                                           aaGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTaa              >  1:431512/1‑62 (MQ=255)                                           aaGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTaa              >  1:4521836/1‑62 (MQ=255)                                           aaGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTaa              >  1:3456133/1‑62 (MQ=255)                                           aaGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTaa              >  1:577403/1‑62 (MQ=255)                                           aaGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTa               >  1:1000585/1‑61 (MQ=255)                                                       gACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAac  >  1:3996059/1‑62 (MQ=255)                                                       gACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAac  >  1:2581340/1‑62 (MQ=255)                                                            |                                                         GATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAAC  >  W3110S.gb/4561031‑4561146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑