| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 527,659 | A→G | K163E (AAA→GAA) | ylbH → | conserved hypothetical protein, rhs‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 527,659 | 0 | A | G | 100.0% | 27.8 / NA | 9 | K163E (AAA→GAA) | ylbH | conserved hypothetical protein, rhs‑like |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/9); total (0/9) | |||||||||||
GTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAA > W3110S.gb/527626‑527709 | gtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATc < 1:3985410/61‑1 (MQ=255)gtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCt < 1:1037032/62‑1 (MQ=255)gtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCt < 1:3222579/62‑1 (MQ=255)gtTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCt < 1:539594/62‑1 (MQ=255) cGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATc < 1:1310220/51‑1 (MQ=255) cGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATc < 1:1355732/51‑1 (MQ=255) cGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATc < 1:3336192/51‑1 (MQ=255) tGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGaaaa < 1:222711/62‑1 (MQ=255) atATGAAGAAGAGATTAGAGATTTCGGGTTAAATc < 1:2952999/34‑1 (MQ=25) | GTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAA > W3110S.gb/527626‑527709 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |