Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,606,089	G→A	N37N (AAC→AAT)	dnaT ←	DNA biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,606,089	0	G	A	100.0%	76.7 / NA	25	N37N (AAC→AAT)	dnaT	DNA biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/3);  total (22/3)

CCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGT  >  W3110S.gb/4606049‑4606141                                         |                                                     ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac                                  >  1:713215/1‑61 (MQ=255) ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac                                  >  1:1616761/1‑61 (MQ=255) ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac                                  >  1:4689873/1‑61 (MQ=255) ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac                                  >  1:1754160/1‑61 (MQ=255) ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac                                  >  1:2243757/1‑61 (MQ=255) ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac                                  >  1:2388459/1‑61 (MQ=255) ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac                                  >  1:2464490/1‑61 (MQ=255) ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac                                  >  1:2621894/1‑61 (MQ=255) ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac                                  >  1:3783471/1‑61 (MQ=255) ccAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACcac                                  >  1:3653684/1‑61 (MQ=255)     gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTATTAGCaaa                                              >  1:4430784/1‑45 (MQ=255)     gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGc                             >  1:3958802/1‑62 (MQ=255)     gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGc                             >  1:2215748/1‑62 (MQ=255)     gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGc                             >  1:2669748/1‑62 (MQ=255)     gcgtgcaggcgtgACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGc                             >  1:2702128/1‑62 (MQ=255)               gtgACGGCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGcc                            <  1:851943/53‑1 (MQ=255)                    ggCATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGcc               <  1:1581000/61‑1 (MQ=255)                      cATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGa            >  1:2944698/1‑62 (MQ=255)                      cATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGa            >  1:2063824/1‑62 (MQ=255)                      cATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGa            >  1:4138588/1‑62 (MQ=255)                      cATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGa            >  1:1916613/1‑62 (MQ=255)                      cATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGa            >  1:447078/1‑62 (MQ=255)                      cATAAAACGCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGa            >  1:47055/1‑62 (MQ=255)                              gCCGGGGCATTATTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTg    <  1:1715875/62‑1 (MQ=255)                                 ggggCATTATTAGCAAATACGGCAACCACACCGCCATCAGCTTTTGCCAGAGCGGTTTGGt  >  1:1123577/1‑61 (MQ=255)                                         |                                                     CCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGT  >  W3110S.gb/4606049‑4606141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑