Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 548,801 G→A pseudogene (1221/1259 nt)
pseudogene (964/1002 nt)
ylbE →
ylbE →
ECK0512:JW0508+JW0507:b4572; hypothetical protein
ECK0512:JW0508:b0519; hypothetical protein, C‑ter fragment

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb548,8010GA100.0% 244.7 / NA 74pseudogene (1221/1259 nt)
pseudogene (964/1002 nt)
ylbE
ylbE
ECK0512:JW0508+JW0507:b4572; hypothetical protein
ECK0512:JW0508:b0519; hypothetical protein, C‑ter fragment
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/45);  total (29/45)

CGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCA  >  W3110S.gb/548742‑548859
                                                           |                                                          
cGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGTCACCGCTGGCGTGCTTTGAACAGacg                                                          <  1:1478009/62‑1 (MQ=255)
cGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacg                                                          <  1:4382594/62‑1 (MQ=255)
cGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacg                                                          <  1:2327615/62‑1 (MQ=255)
cGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacg                                                          <  1:1315965/62‑1 (MQ=255)
cGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacg                                                          <  1:3940748/62‑1 (MQ=255)
cGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacg                                                          <  1:116556/62‑1 (MQ=255)
cGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacg                                                          <  1:3990067/62‑1 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         >  1:1563209/1‑62 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         >  1:2725975/1‑62 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         >  1:2882689/1‑62 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         >  1:3213521/1‑62 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         >  1:3266776/1‑62 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         >  1:1689319/1‑62 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         >  1:3282677/1‑62 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         >  1:3743502/1‑62 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         >  1:446682/1‑62 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         >  1:965081/1‑62 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         <  1:470465/62‑1 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         >  1:4223275/1‑62 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         >  1:1456423/1‑62 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         >  1:1259335/1‑62 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         <  1:4139297/62‑1 (MQ=255)
 ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         <  1:1256532/62‑1 (MQ=255)
 ggggAACGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGacgc                                                         >  1:990239/1‑62 (MQ=255)
             gATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTgg                                             <  1:1381467/62‑1 (MQ=255)
                           ccGTGCGGGCACCGCTGGCGTGCTTTGAACAGacg                                                          >  1:972990/1‑35 (MQ=255)
                           ccGTGCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGaaa                                       >  1:2842492/1‑54 (MQ=255)
                           ccGTGCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGt                               >  1:562938/1‑62 (MQ=255)
                           ccGTGCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGt                               >  1:2891008/1‑62 (MQ=255)
                               gCGGTCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTg                           <  1:2001851/62‑1 (MQ=255)
                               gCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTg                           <  1:544772/62‑1 (MQ=255)
                               gCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTg                           <  1:355975/62‑1 (MQ=255)
                               gCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTg                           <  1:1015789/62‑1 (MQ=255)
                               gCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTg                           <  1:1580049/62‑1 (MQ=255)
                               gCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTg                           <  1:1920917/62‑1 (MQ=255)
                               gCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTg                           <  1:1111272/62‑1 (MQ=255)
                               gCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTg                           <  1:1579170/62‑1 (MQ=255)
                               gCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTg                           <  1:1112257/62‑1 (MQ=255)
                                cGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTgg                          >  1:4265584/1‑62 (MQ=255)
                                cGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTg                           >  1:1062971/1‑61 (MQ=255)
                                cGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTg                           <  1:4093506/61‑1 (MQ=255)
                                    cACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg                       >  1:3626017/1‑61 (MQ=255)
                                    cACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg                       >  1:48350/1‑61 (MQ=255)
                                    cACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg                       >  1:2005333/1‑61 (MQ=255)
                                    cACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg                       >  1:3297569/1‑61 (MQ=255)
                                    cACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg                       >  1:926759/1‑61 (MQ=255)
                                    cACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg                       >  1:3572838/1‑61 (MQ=255)
                                    cACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg                       >  1:281401/1‑61 (MQ=255)
                                    cACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg                       >  1:3780319/1‑61 (MQ=255)
                                    cACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg                       >  1:2664801/1‑61 (MQ=255)
                                    cACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg                       >  1:3663910/1‑61 (MQ=255)
                                    cACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg                       >  1:2519113/1‑61 (MQ=255)
                                    cACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg                       >  1:1028919/1‑61 (MQ=255)
                                    cACCGCTAGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTg                       >  1:1206227/1‑61 (MQ=255)
                                      ccGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTgg                                             <  1:1548144/37‑1 (MQ=255)
                                      ccGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTgg                                             <  1:3809346/37‑1 (MQ=255)
                                      ccGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTgg                                             <  1:3254269/37‑1 (MQ=255)
                                      ccGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTgg                                             <  1:3155284/37‑1 (MQ=255)
                                      ccGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTgg                                             <  1:3028076/37‑1 (MQ=255)
                                      ccGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTgg                                             <  1:2235266/37‑1 (MQ=255)
                                      ccGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTgg                                             <  1:2236381/37‑1 (MQ=255)
                                      ccGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTgg                                             <  1:2726992/37‑1 (MQ=255)
                                      ccGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTgg                                             <  1:627729/37‑1 (MQ=255)
                                      ccGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTgg                                             <  1:25461/37‑1 (MQ=255)
                                         cTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAAc                 <  1:2446402/62‑1 (MQ=255)
                                         cTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAAc                 <  1:1365309/62‑1 (MQ=255)
                                         cTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAAc                 <  1:3014811/62‑1 (MQ=255)
                                          tGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAAc                 <  1:4673471/61‑1 (MQ=255)
                                               tGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:3946064/62‑1 (MQ=255)
                                               tGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:2188659/62‑1 (MQ=255)
                                               tGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:2700304/62‑1 (MQ=255)
                                               tGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:570641/62‑1 (MQ=255)
                                               tGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:2403888/62‑1 (MQ=255)
                                                gCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:4003160/61‑1 (MQ=255)
                                                   ttGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGAc       <  1:3649734/62‑1 (MQ=255)
                                                   ttGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGAc       <  1:3644288/62‑1 (MQ=255)
                                                   ttGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGAc       <  1:2984240/62‑1 (MQ=255)
                                                   ttGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGAc       <  1:4473752/62‑1 (MQ=255)
                                                    tGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAAc                 <  1:575721/51‑1 (MQ=255)
                                                    tGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:994418/57‑1 (MQ=255)
                                                    tGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:2462120/57‑1 (MQ=255)
                                                    tGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:1024808/57‑1 (MQ=255)
                                                    tGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:1326129/57‑1 (MQ=255)
                                                    tGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:4365226/57‑1 (MQ=255)
                                                    tGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:2585865/57‑1 (MQ=255)
                                                    tGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:3289147/57‑1 (MQ=255)
                                                    tGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:3970998/57‑1 (MQ=255)
                                                    tGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCaa           <  1:4347491/57‑1 (MQ=255)
                                                        cAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGCCGatca  >  1:2010695/1‑62 (MQ=255)
                                                        cAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatca  >  1:3778784/1‑62 (MQ=255)
                                                        cAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatca  >  1:3101840/1‑62 (MQ=255)
                                                        cAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatca  >  1:2320908/1‑62 (MQ=255)
                                                        cAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatca  >  1:728796/1‑62 (MQ=255)
                                                        cAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatca  >  1:806698/1‑62 (MQ=255)
                                                        cAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatca  >  1:4419355/1‑62 (MQ=255)
                                                        cAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatc   >  1:4037226/1‑61 (MQ=255)
                                                        cAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatc   >  1:2284343/1‑61 (MQ=255)
                                                        cAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatc   >  1:1408684/1‑61 (MQ=255)
                                                        cAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGat    >  1:1155654/1‑60 (MQ=255)
                                                           |                                                          
CGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCA  >  W3110S.gb/548742‑548859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: