Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	67,598	C→T	G247D (GGC→GAC)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	67,598	0	C	T	100.0%	228.8 / NA	68	G247D (GGC→GAC)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (48/20);  total (48/20)

GAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCG  >  W3110S.gb/67540‑67655                                                           |                                                          gAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGt                                                        <  1:1110068/62‑1 (MQ=255) gAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGt                                                        <  1:4172305/62‑1 (MQ=255)        ttCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTtg                                                 >  1:112594/1‑62 (MQ=255)        ttCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTtg                                                 >  1:901192/1‑62 (MQ=255)        ttCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTtg                                                 >  1:3258061/1‑62 (MQ=255)        ttCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTtg                                                 >  1:3590820/1‑62 (MQ=255)          cATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTtgtg                                               >  1:231069/1‑62 (MQ=255)          cATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTtgtg                                               >  1:2551389/1‑62 (MQ=255)          cATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTtgtg                                               >  1:1488643/1‑62 (MQ=255)          cATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTGTTTTGTCGTGGATTtgtg                                               >  1:3817138/1‑62 (MQ=255)                     cAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTc                                    >  1:3775289/1‑62 (MQ=255)                          cgcgCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGgt                               <  1:178222/62‑1 (MQ=255)                          cgcgCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGgt                               <  1:2682991/62‑1 (MQ=255)                          cgcgCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGgt                               <  1:2009235/62‑1 (MQ=255)                          cgcgCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGgt                               <  1:170560/62‑1 (MQ=255)                          cgcgCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGgt                               <  1:881075/62‑1 (MQ=255)                          cgcgCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGgt                               <  1:1370435/62‑1 (MQ=255)                          cgcgCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGgt                               <  1:1168650/62‑1 (MQ=255)                          cgcgCCGCTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGgt                               <  1:990271/62‑1 (MQ=255)                          cgcgCCGCTTCCAGCACGTTCTGTCGTTCTTTGTCGTGGATTTGTGTGGCAGGCGTCATGgt                               <  1:4594389/62‑1 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTtgtgt                                              >  1:1060823/1‑41 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTtgtgt                                              >  1:657314/1‑41 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTtgtgt                                              >  1:2872711/1‑41 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTtgtgt                                              >  1:959601/1‑41 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagca                         >  1:283161/1‑62 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagca                         <  1:3362891/62‑1 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagca                         >  1:331420/1‑62 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagca                         >  1:451289/1‑62 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagca                         <  1:3578386/62‑1 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagca                         >  1:2746930/1‑62 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagca                         >  1:1659788/1‑62 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagca                         >  1:93992/1‑62 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagca                         >  1:4129392/1‑62 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagca                         <  1:2556259/62‑1 (MQ=255)                                gcTTCCAGCACGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagca                         >  1:2387795/1‑62 (MQ=255)                                gcTTCCAGCACGTTCTGTCGGTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagc                          >  1:612159/1‑61 (MQ=255)                                          cGTTCTGTCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGgtg                              <  1:4197314/47‑1 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGgtgt                             >  1:3450966/1‑41 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:4598607/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:4158049/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:384936/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:1837940/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:1947280/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:518420/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:639803/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:958700/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:1239471/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:1018540/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:3651440/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:2518270/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:2566354/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:2066755/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:3075508/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:3252341/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:3449874/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:1948079/1‑47 (MQ=255)                                                 tCGTTTTTTGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTagcagc                       >  1:1901256/1‑47 (MQ=255)                                                     tttttGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCa     <  1:1835811/61‑1 (MQ=255)                                                     tttttGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCa     <  1:3360373/61‑1 (MQ=255)                                                     tttttGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCa     <  1:620158/61‑1 (MQ=255)                                                     tttttGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCa     <  1:2298724/61‑1 (MQ=255)                                                      ttttGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAgc   <  1:2071514/62‑1 (MQ=255)                                                       tttGTCGTGGATTTTTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATAGACCAgcg  >  1:1995557/1‑62 (MQ=255)                                                       tttGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAgcg  >  1:3284133/1‑62 (MQ=255)                                                       tttGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAgcg  >  1:627033/1‑62 (MQ=255)                                                       tttGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAgcg  >  1:1661352/1‑62 (MQ=255)                                                       tttGTCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAgcg  >  1:266618/1‑62 (MQ=255)                                                       tttGTCGTGGATTTGTGTGGCAGGCGTAATGGTGTAGCAGCTTTCGTACTCATCGACCAgcg  >  1:1309872/1‑62 (MQ=255)                                                           |                                                          GAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCG  >  W3110S.gb/67540‑67655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑