Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	556,858	A→T	L36Q (CTG→CAG)	folD ←	bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	556,858	0	A	T	100.0%	48.2 / NA	16	L36Q (CTG→CAG)	folD	bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/5);  total (11/5)

ATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCA  >  W3110S.gb/556815‑556914                                            |                                                         aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTc                                        <  1:1542567/62‑1 (MQ=255) aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTc                                        <  1:2508624/62‑1 (MQ=255) aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTc                                        <  1:3194799/62‑1 (MQ=255)        tGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc                                 >  1:736875/1‑62 (MQ=255)                           ccaccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttt               >  1:1635004/1‑61 (MQ=255)                                 gCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc                                 >  1:1601582/1‑37 (MQ=255)                                 gCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc                                 >  1:1793940/1‑37 (MQ=255)                                 gCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc                                 >  1:1869843/1‑37 (MQ=255)                                 gCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc                                 >  1:2815595/1‑37 (MQ=255)                                 gCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc                                 >  1:3182926/1‑37 (MQ=255)                                 gCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc                                 >  1:3829512/1‑37 (MQ=255)                                 gCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc                                 >  1:4099152/1‑37 (MQ=255)                                 gCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc                                 >  1:4295900/1‑37 (MQ=255)                                       cGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt    >  1:2115692/1‑60 (MQ=255)                                       cGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa  <  1:198450/62‑1 (MQ=255)                                       cGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa  <  1:444414/62‑1 (MQ=255)                                            |                                                         ATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCA  >  W3110S.gb/556815‑556914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑