Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	563,782	C→T	intergenic (‑79/‑164)	fimZ ← / → argU	predicted DNA‑binding transcriptional regulator/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	563,782	0	C	T	100.0%	99.5 / NA	31	intergenic (‑79/‑164)	fimZ/argU	predicted DNA‑binding transcriptional regulator/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/15);  total (16/15)

CGGACAATTTTCCGTTAGGTCGGGAAATTGTACTTTGATACATGAAAATACGGGTTTTCTTGATTCAGACGCGCAGCGGTGTGCGTTTGTTTGCCGCTATAGCGAAATAAA  >  W3110S.gb/563724‑563834                                                           |                                                     cGGACAATTTTCCGTTAGGTCGGGAAATTGTACTTTGATACATGAAAATACGGGTTTTTTTg                                                   <  1:2733752/62‑1 (MQ=255) cGGACAATTTTCCGTTAGGTCGGGAAATTGTACTTTGATACATGAAAATACGGGTTTTTTTg                                                   <  1:655248/62‑1 (MQ=255)                     cGGGAAATTGTACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGgtgt                               <  1:3601746/62‑1 (MQ=255)                     cGGGAAATTGTACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGgtgt                               <  1:1360980/62‑1 (MQ=255)                     cGGGAAATTGTACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGgtgt                               <  1:4437108/62‑1 (MQ=255)                     cGGGAAATTGTACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGgtgt                               <  1:1577977/62‑1 (MQ=255)                     cGGGAAATTGTACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGgtgt                               <  1:3426849/62‑1 (MQ=255)                     cGGGAAATTGTACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGgtgt                               <  1:201095/62‑1 (MQ=255)                     cGGGAAATTGTACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGgtgt                               <  1:3226575/62‑1 (MQ=255)                     cGGGAAATTGTACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGgtgt                               <  1:2415034/62‑1 (MQ=255)                     cGGGAAATTGTACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGgtgt                               <  1:2952955/62‑1 (MQ=255)                            ttGTACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCgtttg                        >  1:1241404/1‑62 (MQ=255)                            ttGTACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCgtttg                        >  1:1781555/1‑62 (MQ=255)                            ttGTACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCgtttg                        >  1:2005301/1‑62 (MQ=255)                              gTACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGgtgt                               <  1:4036594/53‑1 (MQ=255)                               tACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCgtttgttt                     >  1:3056597/1‑62 (MQ=255)                               tACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCgtttgttt                     >  1:4615564/1‑62 (MQ=255)                               tACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCgtttgttt                     >  1:691372/1‑62 (MQ=255)                               tACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCgtttgttt                     >  1:1025449/1‑62 (MQ=255)                               tACTTTGATACATGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCgtttgttt                     >  1:4185919/1‑62 (MQ=255)                                           tGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGGTTTGCGTTTGTTTGCCGCTATAGc          >  1:48707/1‑61 (MQ=255)                                           tGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCGTTTGTTTGCCGCTATAg           >  1:2165594/1‑60 (MQ=255)                                           tGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCGTTTGTTTGCCGCTATAGc          >  1:688022/1‑61 (MQ=255)                                           tGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCGTTTGTTTGCCGCTATAGc          >  1:1034354/1‑61 (MQ=255)                                           tGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCGTTTGTTTGCCGCTATAGc          >  1:4015145/1‑61 (MQ=255)                                           tGAAAATACGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCGTTTGTTTGCCGCTATAGc          >  1:2007635/1‑61 (MQ=255)                                                  aCGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCGTTTGTTTGCCGc                >  1:3210132/1‑48 (MQ=255)                                                  aCGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCGTTTGTTTGCCGCTATAGCGaaataaa  <  1:3587201/62‑1 (MQ=255)                                                  aCGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCGTTTGTTTGCCGCTATAGCGaaataaa  <  1:2966377/62‑1 (MQ=255)                                                  aCGGGTTTTTTTGATTCAGACGCGCAGCGGTGTGCGTTTGTTTGCCGCTATAGCGaaataaa  <  1:2747068/62‑1 (MQ=255)                                                    gggTTTTTTTGATTCAGACGCGCAGCGGTGTGCGTTTGTTTGCCGCTATAGCGaaataa   >  1:1396758/1‑59 (MQ=255)                                                           |                                                     CGGACAATTTTCCGTTAGGTCGGGAAATTGTACTTTGATACATGAAAATACGGGTTTTCTTGATTCAGACGCGCAGCGGTGTGCGTTTGTTTGCCGCTATAGCGAAATAAA  >  W3110S.gb/563724‑563834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑