| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 593,201 | A→G | F265L (TTC→CTC) | cusS ← | sensory histidine kinase in two‑component regulatory system with CusR, senses copper ions |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 593,201 | 0 | A | G | 100.0% | 266.4 / NA | 71 | F265L (TTC→CTC) | cusS | sensory histidine kinase in two‑component regulatory system with CusR, senses copper ions |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (47/24); total (47/24) | |||||||||||
GGTTTGCGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGAC > W3110S.gb/593142‑593256 | ggTTTGCGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGAt > 1:2966831/1‑62 (MQ=255)ggTTTGCGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGAt > 1:3992607/1‑62 (MQ=255) tGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCg > 1:4509909/1‑61 (MQ=255) tGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCg > 1:3952376/1‑61 (MQ=255) tGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCg > 1:2612984/1‑61 (MQ=255) tGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCg > 1:670794/1‑61 (MQ=255) tGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCg > 1:758722/1‑61 (MQ=255) tGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCg > 1:4027775/1‑61 (MQ=255) tGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCg > 1:4150304/1‑61 (MQ=255) cGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGa < 1:2851804/62‑1 (MQ=255) cGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGa < 1:2722031/62‑1 (MQ=255) cGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGa < 1:243405/62‑1 (MQ=255) cGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGa < 1:2480065/62‑1 (MQ=255) tGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATcc > 1:2818951/1‑61 (MQ=255) tGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATcc > 1:4007731/1‑61 (MQ=255) tGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATcc > 1:517299/1‑61 (MQ=255) tGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATcc > 1:232302/1‑61 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTgg > 1:1931599/1‑40 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTgg > 1:4304455/1‑40 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTgg > 1:2638750/1‑40 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTgg > 1:3386883/1‑40 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTgg > 1:3700241/1‑40 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTgg > 1:481583/1‑40 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTgg > 1:4617884/1‑40 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTgg > 1:4026432/1‑40 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTgg > 1:363703/1‑40 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTgg > 1:3655940/1‑40 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:3498059/1‑39 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:462303/1‑39 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:3009465/1‑39 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:3645623/1‑39 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:2517107/1‑39 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:2880763/1‑39 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:4509584/1‑39 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:2630186/1‑39 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:2562652/1‑39 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:57644/1‑39 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:743954/1‑39 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:798127/1‑39 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:1833651/1‑39 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:1380377/1‑39 (MQ=255) ggTGTGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:832008/1‑39 (MQ=255) ggTGTGCGAATTTCGAGAGCGATATCCGCTGAGAGATTgg > 1:4334315/1‑40 (MQ=255) ggTGCGCGAATTTCGTGAGCGATATCCGCTGAGAGATTg > 1:717393/1‑39 (MQ=38) cGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTg < 1:3838462/38‑1 (MQ=255) cGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTg < 1:2754419/38‑1 (MQ=255) cGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTg < 1:2669134/38‑1 (MQ=255) cGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTg < 1:2411252/38‑1 (MQ=255) cGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTg < 1:182339/38‑1 (MQ=255) cGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTg < 1:1400590/38‑1 (MQ=255) cGAATTTCGTGAGCGATATCCGCTGAGAGATTGGACTg < 1:1243807/38‑1 (MQ=255) cGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTa > 1:739720/1‑38 (MQ=255) cGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTa > 1:950293/1‑38 (MQ=255) cGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTa > 1:1145905/1‑38 (MQ=255) cGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTa > 1:389055/1‑38 (MQ=255) cGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTa > 1:3560644/1‑38 (MQ=255) cGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTa > 1:2029323/1‑38 (MQ=255) cGTGAGCGATATCCGCTGAGAGATTGGACTGGCGGGTa > 1:2084753/1‑38 (MQ=255) gAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCata < 1:2151965/62‑1 (MQ=255) gAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCata < 1:1049589/62‑1 (MQ=255) gAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCata < 1:1859153/62‑1 (MQ=255) gAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCata < 1:2362638/62‑1 (MQ=255) gAGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCata < 1:4433948/62‑1 (MQ=255) aGCGATATCCGCTGAGAGATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCata < 1:2112198/61‑1 (MQ=255) cTGAGAGATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGAc < 1:2211483/62‑1 (MQ=255) cTGAGAGATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGAc < 1:4326932/62‑1 (MQ=255) cTGAGAGATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGAc < 1:2218520/62‑1 (MQ=255) cTGAGAGATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGAc < 1:3540778/62‑1 (MQ=255) cTGAGAGATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGAc < 1:829329/62‑1 (MQ=255) cTGAGAGATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGAc < 1:3973740/62‑1 (MQ=255) tGAGAGATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGAc < 1:2111010/61‑1 (MQ=255) | GGTTTGCGTTATGAGATTCGTAATTGGTGTGCGAATTTCGTGAGCGATATCCGCTGAGAAATTGGACTGGCGGGTAAAGACATCCTCAATACGCTCGATCATATGGTTGAACGAC > W3110S.gb/593142‑593256 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |