Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	639,164	G→A	P63P (CCG→CCA)	ahpF →	alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	639,164	0	G	A	100.0%	166.8 / NA	52	P63P (CCG→CCA)	ahpF	alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/7);  total (45/7)

GCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGT  >  W3110S.gb/639105‑639206                                                            |                                           gCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATc                                          <  1:2717391/62‑1 (MQ=255) gCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATc                                          <  1:1343321/62‑1 (MQ=255) gCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATc                                          <  1:3981175/62‑1 (MQ=255) gCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATc                                          <  1:4012784/62‑1 (MQ=255)   aGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCtt                                        <  1:3719368/62‑1 (MQ=255)      aCTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTc                                      >  1:2002758/1‑61 (MQ=255)         gTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTGa                                  <  1:1854766/62‑1 (MQ=255)                           tAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCATc                                          <  1:605431/36‑1 (MQ=255)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCt                                         >  1:1832169/1‑35 (MQ=255)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTg                                   >  1:330983/1‑41 (MQ=255)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTg                                   >  1:3813814/1‑41 (MQ=255)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTg                                   >  1:4121495/1‑41 (MQ=255)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTg                                   >  1:4201119/1‑41 (MQ=255)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTg                                   >  1:1619527/1‑41 (MQ=255)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCTg                                   >  1:550810/1‑41 (MQ=255)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg                              >  1:4124989/1‑44 (MQ=38)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg                              >  1:4113302/1‑44 (MQ=38)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg                              >  1:428485/1‑44 (MQ=38)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg                              >  1:450985/1‑44 (MQ=38)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg                              >  1:375286/1‑44 (MQ=38)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg                              >  1:3746373/1‑44 (MQ=38)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg                              >  1:3601471/1‑44 (MQ=38)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg                              >  1:1167804/1‑44 (MQ=38)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg                              >  1:702338/1‑44 (MQ=38)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCCAGATcgg                              >  1:2759817/1‑44 (MQ=38)                             aagaagATAACAGCTTGCCGGTGCGTAAGCCATCTTTCAAGATcg                               >  1:2126314/1‑44 (MQ=37)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa                >  1:1220764/1‑48 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa                >  1:1233591/1‑48 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa                >  1:4269178/1‑48 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa                >  1:2074665/1‑48 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa                >  1:2241341/1‑48 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa                >  1:3978238/1‑48 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa                >  1:3100105/1‑48 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa                >  1:2285835/1‑48 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCa                >  1:2399774/1‑48 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACg   >  1:219852/1‑61 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGt  >  1:273176/1‑62 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGt  >  1:2555469/1‑62 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGt  >  1:3764696/1‑62 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGt  >  1:1691977/1‑62 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGt  >  1:1768027/1‑62 (MQ=255)                                         gCTTGCCGGTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGt  >  1:4457451/1‑62 (MQ=255)                                                ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc             >  1:3464039/1‑44 (MQ=255)                                                ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc             >  1:1331299/1‑44 (MQ=255)                                                ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc             >  1:4597964/1‑44 (MQ=255)                                                ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc             >  1:4568443/1‑44 (MQ=255)                                                ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc             >  1:4276531/1‑44 (MQ=255)                                                ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc             >  1:1937280/1‑44 (MQ=255)                                                ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc             >  1:195727/1‑44 (MQ=255)                                                ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc             >  1:2714060/1‑44 (MQ=255)                                                ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc             >  1:3984092/1‑44 (MQ=255)                                                ggTGCGTAAGCCATCTTTCCTGATCACCAACCCAGGTTCCAAcc             >  1:227015/1‑44 (MQ=255)                                                            |                                           GCAGAACTGTCAGACAAAGTCACCTTTAAAGAAGATAACAGCTTGCCGGTGCGTAAGCCGTCTTTCCTGATCACCAACCCAGGTTCCAACCAGGGGCCACGT  >  W3110S.gb/639105‑639206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑