Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	647,863	A→G	M311T (ATG→ACG)	citF ←	citrate lyase, citrate‑ACP transferase (alpha) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	647,863	0	A	G	100.0%	189.1 / NA	51	M311T (ATG→ACG)	citF	citrate lyase, citrate‑ACP transferase (alpha) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/17);  total (34/17)

AAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAA  >  W3110S.gb/647808‑647891                                                        |                             aaaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcgtcg                        >  1:3406068/1‑62 (MQ=255) aaaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcgtcg                        >  1:3310298/1‑62 (MQ=255) aaaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcgtcg                        >  1:3258094/1‑62 (MQ=255) aaaGCTCTGCACATCCAGCAGTTTACGGATCAGACCTTTTTCGTGCAGGTCAACCGtcgtcg                        >  1:3368935/1‑62 (MQ=255)  aaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc                       >  1:3525537/1‑62 (MQ=255)  aaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc                       >  1:3256263/1‑62 (MQ=255)  aaGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc                       >  1:2434274/1‑62 (MQ=255)   aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc                       <  1:3412799/61‑1 (MQ=255)   aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc                       <  1:901314/61‑1 (MQ=255)   aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc                       <  1:4467301/61‑1 (MQ=255)   aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc                       <  1:1545034/61‑1 (MQ=255)   aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc                       <  1:1599440/61‑1 (MQ=255)   aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc                       <  1:2505993/61‑1 (MQ=255)   aGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTcgc                       <  1:2500456/61‑1 (MQ=255)       ctGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTaa                  <  1:1732441/62‑1 (MQ=255)       ctGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTaa                  <  1:4464531/62‑1 (MQ=255)       ctGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTaa                  <  1:14929/62‑1 (MQ=255)       ctGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTaa                  <  1:683782/62‑1 (MQ=255)                 agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt                    >  1:4139307/1‑50 (MQ=255)                 agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt                    >  1:3096571/1‑50 (MQ=255)                 agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt                    >  1:1226193/1‑50 (MQ=255)                 agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt                    >  1:4376200/1‑50 (MQ=255)                 agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt                    >  1:2695201/1‑50 (MQ=255)                 agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt                    >  1:4663560/1‑50 (MQ=255)                 agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt                    >  1:468199/1‑50 (MQ=255)                 agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt                    >  1:2224124/1‑50 (MQ=255)                 agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt                    >  1:4834/1‑50 (MQ=255)                 agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt                    >  1:563160/1‑50 (MQ=255)                 agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt                    >  1:1304973/1‑50 (MQ=255)                 agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt                    >  1:1251497/1‑50 (MQ=255)                      tttGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt                    >  1:725107/1‑45 (MQ=255)                       ttGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcg                           <  1:4077949/37‑1 (MQ=255)                       ttGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcg                           <  1:1233483/37‑1 (MQ=255)                       ttGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcg                           <  1:4046151/37‑1 (MQ=255)                       ttGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcg                           <  1:235928/37‑1 (MQ=255)                       ttGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcg                           <  1:2395832/37‑1 (MQ=255)                       ttGCGGATCAGACCTTTTTCATGCAGGTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGaa  >  1:4511858/1‑62 (MQ=255)                        tGCGGATCAGACCTTTTTCGTGCAGGTCAACcgtcgt                          <  1:688365/37‑1 (MQ=255)                        tGCGGATCAGACCTTTTTCGTGCAGGTCAACcgtcgt                          <  1:1359271/37‑1 (MQ=255)                        tGCGGATCAGACCTTTTTCGTGCAGGTCAACcgtcgt                          <  1:600599/37‑1 (MQ=255)                        tGCGGATCAGACCTTTTTCGTGCAGGTCAACcgtcgt                          <  1:2591632/37‑1 (MQ=255)                        tGCGGATCAGACCTTTTTCGTGCAGGTCAACCGtcg                           <  1:914149/36‑1 (MQ=255)                              tCAGACCTTTTTCGTGCAGGTCAGCCGTCGTCGCGGt                    >  1:4299941/1‑37 (MQ=37)                              tCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCgg                     >  1:1271960/1‑36 (MQ=255)                              tCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt                    >  1:3072203/1‑37 (MQ=255)                               cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa                   >  1:4490172/1‑37 (MQ=255)                               cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa                   >  1:2636737/1‑37 (MQ=255)                               cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa                   >  1:2924235/1‑37 (MQ=255)                               cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa                   >  1:2119040/1‑37 (MQ=255)                               cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa                   >  1:1535913/1‑37 (MQ=255)                               cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa                   >  1:3162492/1‑37 (MQ=255)                               cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa                   >  1:3742525/1‑37 (MQ=255)                               cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa                   >  1:713885/1‑37 (MQ=255)                               cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa                   >  1:1284170/1‑37 (MQ=255)                               cAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTa                   >  1:3922328/1‑37 (MQ=255)                                                        |                             AAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAA  >  W3110S.gb/647808‑647891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑