Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	651,069	T→C	N4S (AAT→AGT)	citC ←	citrate lyase synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	651,069	0	T	C	100.0%	148.9 / NA	41	N4S (AAT→AGT)	citC	citrate lyase synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/34);  total (7/34)

CGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAT  >  W3110S.gb/651016‑651127                                                      |                                                           cGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCACTGCCGaa                                                     <  1:876641/61‑1 (MQ=255) cGATTTCCGCCATTTTTTTATTTTCTGAACGTCTTACGCGGGTGAAAATATCACTGCCGaa                                                     <  1:2751599/61‑1 (MQ=255)  gATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCACTGCCGaa                                                     <  1:2402396/60‑1 (MQ=255)             tttttttATTTTCTGAACGTTTTACGCGGGTGAAAATATCACTGCCGAACATAATAAATAGt                                        >  1:2582491/1‑62 (MQ=255)                                       cgGGTGAAAATATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGa              >  1:1237022/1‑62 (MQ=255)                                       cgGGTGAAAATATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGa              >  1:2664232/1‑62 (MQ=255)                                       cgGGTGAAAATATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGa              >  1:862361/1‑62 (MQ=255)                                         ggTGAAAATATCACTGCCGAACATAATAAATAGTATCCTGaa                                >  1:519941/1‑42 (MQ=255)                                         ggTGAAAATATCACTGCCGAACATAATAAATAGTATCCTGaa                                >  1:3699578/1‑42 (MQ=255)                                         ggTGAAAATATCACTGCCGAACATAATAAATAGTATCCTGaa                                >  1:482560/1‑42 (MQ=255)                                         ggTGAAAATATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAtt            <  1:4508618/62‑1 (MQ=255)                                             aaaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:4686805/48‑1 (MQ=255)                                             aaaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:3006130/48‑1 (MQ=255)                                             aaaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:846460/48‑1 (MQ=255)                                             aaaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:4603535/48‑1 (MQ=255)                                             aaaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:4263276/48‑1 (MQ=255)                                             aaaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:4342588/48‑1 (MQ=255)                                             aaaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:455812/48‑1 (MQ=255)                                             aaaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:707285/48‑1 (MQ=255)                                             aaaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:2314366/48‑1 (MQ=255)                                             aaaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:2189798/48‑1 (MQ=255)                                             aaaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:2165804/48‑1 (MQ=255)                                             aaaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:1777808/48‑1 (MQ=255)                                             aaaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:1508341/48‑1 (MQ=255)                                             aaaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:120059/48‑1 (MQ=255)                                              aaaTATCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgtt                      <  1:851457/47‑1 (MQ=255)                                                atatCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGaat            <  1:2100404/55‑3 (MQ=255)                                                atatCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAtt            <  1:942740/55‑1 (MQ=255)                                                atatCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAtt            <  1:1006845/55‑1 (MQ=255)                                                atatCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAtt            <  1:4128778/55‑1 (MQ=255)                                                atatCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAtt            <  1:3194328/55‑1 (MQ=255)                                                atatCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAtt            <  1:2957648/55‑1 (MQ=255)                                                atatCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAtt            <  1:2720238/55‑1 (MQ=255)                                                atatCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAtt            <  1:2484449/55‑1 (MQ=255)                                                atatCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAtt            <  1:2084566/55‑1 (MQ=255)                                                atatCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAtt            <  1:172017/55‑1 (MQ=255)                                                atatCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAtt            <  1:1048865/55‑1 (MQ=255)                                                   tCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAtt            <  1:730829/52‑1 (MQ=255)                                                   tCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAt  <  1:4071941/62‑1 (MQ=255)                                                   tCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAt  <  1:814312/62‑1 (MQ=255)                                                   tCACTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAt  <  1:1302139/62‑1 (MQ=255)                                                      |                                                           CGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAT  >  W3110S.gb/651016‑651127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑