Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	707,707	A→G	N398S (AAC→AGC)	glnS →	glutamyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	707,707	0	A	G	100.0%	108.9 / NA	30	N398S (AAC→AGC)	glnS	glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/11);  total (19/11)

TAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAAT  >  W3110S.gb/707660‑707756                                                |                                                  tAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAAc                                     <  1:1519053/62‑1 (MQ=255) tAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAAc                                     <  1:1765992/62‑1 (MQ=255) tAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAAc                                     <  1:4425223/62‑1 (MQ=255) tAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAAc                                     <  1:3221747/62‑1 (MQ=255) tAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAAc                                     <  1:2152153/62‑1 (MQ=255)  aGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAAc                                     <  1:1052525/61‑1 (MQ=255)  aGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAAc                                     <  1:4570754/61‑1 (MQ=255)           aTTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCt                           <  1:951151/62‑1 (MQ=255)           aTTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCt                           <  1:3218531/62‑1 (MQ=255)            tttGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAg                                            >  1:3823282/1‑44 (MQ=255)            tttGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTg                          >  1:2857603/1‑62 (MQ=255)            tttGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTg                          >  1:2362343/1‑62 (MQ=255)            tttGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTg                          >  1:2702124/1‑62 (MQ=255)            tttGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTg                          >  1:4332523/1‑62 (MQ=255)            tttGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTg                          >  1:4580568/1‑62 (MQ=255)            tttGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTg                          >  1:4537644/1‑62 (MQ=255)            tttGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGAGCTg                          >  1:652267/1‑62 (MQ=255)                gATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACa                                        >  1:10468/1‑44 (MQ=255)                gATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACa                                        >  1:899031/1‑44 (MQ=255)                gATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACa                                        >  1:845415/1‑44 (MQ=255)                gATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACa                                        >  1:659882/1‑44 (MQ=255)                gATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACa                                        >  1:4440466/1‑44 (MQ=255)                gATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACa                                        >  1:1798699/1‑44 (MQ=255)                gATTGATCGCGCCGATTTCCGCGAAGAAGCTAGCAAGCAGTACa                                        >  1:3330672/1‑44 (MQ=255)                          gcCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTTGTGCTGGGTAAAGAAGTGCg            >  1:8695/1‑62 (MQ=255)                          gcCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCg            >  1:1274083/1‑62 (MQ=255)                          gcCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCg            >  1:4291424/1‑62 (MQ=255)                          gcCGATTTCCGCGAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCg            >  1:2107052/1‑62 (MQ=255)                                     cgAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAAt  <  1:3624842/61‑1 (MQ=255)                                     cgAAGAAGCTAGCAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAAt  <  1:3463463/61‑1 (MQ=255)                                                |                                                  TAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAAT  >  W3110S.gb/707660‑707756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑