BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	711,161	C→T	intergenic (‑93/+196)	fur ← / ← fldA	DNA‑binding transcriptional dual regulator/flavodoxin 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	711,161	0	C	T	100.0%	113.9 / NA	35	intergenic (‑93/+196)	fur/fldA	DNA‑binding transcriptional dual regulator/flavodoxin 1
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/15); total (20/15)

ATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACA  >  W3110S.gb/711103‑711216

aTTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGc                                                       >  1:1467741/1‑61 (MQ=255)

aTTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGc                                                       >  1:230455/1‑61 (MQ=255)

aTTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGc                                                       >  1:2018840/1‑61 (MQ=255)

       gaagTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGa                                               <  1:502605/62‑1 (MQ=255)

                    ttGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTcc                                  >  1:587066/1‑62 (MQ=255)

                    ttGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTcc                                  >  1:174915/1‑62 (MQ=255)

                    ttGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTcc                                  >  1:2658229/1‑62 (MQ=255)

                    ttGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTcc                                  >  1:259486/1‑62 (MQ=255)

                    ttGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTcc                                  >  1:2084688/1‑62 (MQ=255)

                    ttGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTcc                                  >  1:1898569/1‑62 (MQ=255)

                     tGCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTccc                                 <  1:847817/62‑1 (MQ=255)

                      gCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCt                                >  1:2022350/1‑62 (MQ=255)

                      gCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCt                                >  1:462480/1‑62 (MQ=255)

                      gCTCTTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCt                                >  1:4525279/1‑62 (MQ=255)

                         ctTGAGATAATGCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTc                                   >  1:3452888/1‑56 (MQ=255)

                                    gCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTta                  <  1:1572557/62‑1 (MQ=255)

                                    gCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTta                  <  1:720043/62‑1 (MQ=255)

                                    gCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTta                  <  1:3810874/62‑1 (MQ=255)

                                    gCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTta                  <  1:2327305/62‑1 (MQ=255)

                                    gCGTATCATTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTta                  <  1:1529464/62‑1 (MQ=255)

                                           aTTATAGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATaaaaa           >  1:2872985/1‑62 (MQ=255)

                                             tataGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACg         <  1:3559151/62‑1 (MQ=255)

                                              ataGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACg         <  1:160651/61‑1 (MQ=255)

                                                aGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTgg      >  1:3415989/1‑62 (MQ=255)

                                                aGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTgg      >  1:622733/1‑62 (MQ=255)

                                                aGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTgg      >  1:415984/1‑62 (MQ=255)

                                                aGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTgg      >  1:2191168/1‑62 (MQ=255)

                                                aGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTgg      >  1:1877335/1‑62 (MQ=255)

                                                aGAATTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTgg      >  1:1610479/1‑62 (MQ=255)

                                                  aaTTGCCATGCCTAAATGAAAACCACAAGTCCCTGGC‑AATATTGTTAATAAAAACGTGGca    <  1:1952423/61‑1 (MQ=39)

                                                   aTTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGca    <  1:1870601/61‑1 (MQ=255)

                                                   aTTGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGca    <  1:1781224/61‑1 (MQ=255)

                                                    ttGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGcaca  <  1:3971298/62‑1 (MQ=255)

                                                    ttGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGcaca  <  1:1264958/62‑1 (MQ=255)

                                                     tGCCATGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGcaca  <  1:2275034/61‑1 (MQ=255)

ATTAGAAGAAGTGACAGAATTTGCTCTTGAGATAATGCGTATCATTATAGAATTGCCACGCCTAAATGAAAACCACAAGTCCCTGGCAAATATTGTTAATAAAAACGTGGCACA  >  W3110S.gb/711103‑711216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`