Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	811,947	G→T	G382V (GGC→GTC)  A2S (GCA→TCA)	bioF → bioC →	8‑amino‑7‑oxononanoate synthase predicted methltransferase, enzyme of biotin synthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	811,947	0	G	T	100.0%	34.7 / NA	12	G382V (GGC→GTC)  A2S (GCA→TCA)	bioF bioC	8‑amino‑7‑oxononanoate synthase predicted methltransferase, enzyme of biotin synthesis
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/0);  total (12/0)

ACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT  >  W3110S.gb/811889‑812003                                                           |                                                         aCGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGTCaa                                                       >  1:3271141/1‑62 (MQ=255) aCGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGTCaa                                                       >  1:4058773/1‑62 (MQ=255)           cTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGTCAACGGTtaata                                              >  1:1070446/1‑61 (MQ=255)           cTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGTCAACGGTtaata                                              >  1:2351341/1‑61 (MQ=255)                                       tCTGCTGGAGGTGCTGCATGTCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCg                  >  1:2222966/1‑61 (MQ=255)                                       tCTGCTGGAGGTGCTGCATGTCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCg                  >  1:2861035/1‑61 (MQ=255)                                       tCTGCTGGAGGTGCTGCATGTCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCg                  >  1:3040293/1‑61 (MQ=255)                                       tCTGCTGGAGGTGCTGCATGTCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCg                  >  1:312866/1‑61 (MQ=255)                                       tCTGCTGGAGGTGCTGCATGTCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCg                  >  1:556850/1‑61 (MQ=255)                                       tCTGCTGGAGGTGCTGCATGTCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCg                  >  1:984811/1‑61 (MQ=255)                                       tCTGCTGCAGGTGCTGCATGTCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGt                    >  1:3877556/1‑59 (MQ=255)                                                       cATGTCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAt  >  1:1023782/1‑61 (MQ=255)                                                           |                                                         ACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTAT  >  W3110S.gb/811889‑812003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑