Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	833,307	G→T	T22T (ACC→ACA)	ybiA ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	833,307	0	G	T	100.0%	85.7 / NA	27	T22T (ACC→ACA)	ybiA	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/19);  total (8/19)

AACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGA  >  W3110S.gb/833256‑833364                                                    |                                                          aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGa                                                 <  1:500352/62‑1 (MQ=255) aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGa                                                 <  1:3194274/62‑1 (MQ=255) aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGa                                                 <  1:439265/62‑1 (MQ=255) aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGa                                                 <  1:2002272/62‑1 (MQ=255) aaCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGa                                                 <  1:192514/62‑1 (MQ=255)          gggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTatg                                         <  1:4012691/61‑1 (MQ=255)          gggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTatg                                         <  1:3816578/61‑1 (MQ=255)           ggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTat                                          <  1:704739/59‑1 (MQ=255)           ggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTat                                          <  1:4196505/59‑1 (MQ=255)           ggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTat                                          <  1:3227061/59‑1 (MQ=255)           ggCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTat                                          <  1:1657342/59‑1 (MQ=255)             cgaGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATgg                                     <  1:1873811/60‑1 (MQ=255)             ccAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATgg                                     <  1:4617793/62‑1 (MQ=255)             ccAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATgg                                     <  1:1076099/62‑1 (MQ=255)             ccAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATgg                                     <  1:3992884/62‑1 (MQ=255)             ccAGGCGGCAAAGTTAGAAAAATCACCGTAGGCATCGCTTGTGCTGTAGAAATTTatgattg                                     <  1:2160294/62‑3 (MQ=255)                   ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGgtgt                                  >  1:638065/1‑59 (MQ=255)                   ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGgtgt                                  >  1:1064228/1‑59 (MQ=255)                   ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGgtgt                                  >  1:1685004/1‑59 (MQ=255)                   ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGgtgt                                  >  1:1618262/1‑59 (MQ=255)                   ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGgtgt                                  >  1:1413622/1‑59 (MQ=255)                                   tCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGAt               >  1:3917655/1‑62 (MQ=255)                                   tCACCGTAGTCATCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGAt               >  1:163427/1‑62 (MQ=255)                                              aTCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTc    <  1:3784683/62‑1 (MQ=255)                                              aTCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTc    <  1:1057299/62‑1 (MQ=255)                                              aTCGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTc    <  1:767712/62‑1 (MQ=255)                                                cGCTTGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGa  >  1:782809/1‑62 (MQ=255)                                                    |                                                          AACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGA  >  W3110S.gb/833256‑833364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑