Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	862,250	G→T	T260N (ACT→AAT)	ybiW ←	predicted pyruvate formate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	862,250	0	G	T	100.0%	48.6 / NA	16	T260N (ACT→AAT)	ybiW	predicted pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/8);  total (8/8)

TAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGT  >  W3110S.gb/862191‑862283                                                            |                                  tAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTc                                 >  1:3401262/1‑62 (MQ=255) tAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTc                                 >  1:39453/1‑62 (MQ=255)     ttCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTgc                              <  1:3878405/61‑1 (MQ=255)     ttCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTgc                              <  1:718244/61‑1 (MQ=255)      tCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTgcgg                            <  1:1795152/62‑1 (MQ=255)      tCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTgcgg                            <  1:4657153/62‑1 (MQ=255)      tCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTgcgg                            <  1:4677372/62‑1 (MQ=255)      tCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTgcgg                            <  1:545155/62‑1 (MQ=255)       cGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTgcgg                            <  1:4036893/61‑1 (MQ=255)             gCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCtgg                     >  1:2735226/1‑62 (MQ=255)             gCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCtgg                     >  1:2931791/1‑62 (MQ=255)             gCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCtgg                     >  1:3011198/1‑62 (MQ=255)             gCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCtgg                     >  1:3947294/1‑62 (MQ=255)                     aaCTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCg               >  1:1257398/1‑60 (MQ=255)                                gTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAg   <  1:1457542/61‑1 (MQ=255)                                gTAACACAGTTGCAGCGCCTGCCAGAAATTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGt  >  1:808759/1‑62 (MQ=255)                                                            |                                  TAGATTCGATCTGCAAAATCAACTGGATGAAGTAACACAGTTGCAGCGCCTGCCAGAAAGTCTGCGGCGGCTGGTGGGCGATAAGATCGCAGT  >  W3110S.gb/862191‑862283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑