Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	891,153	C→T	R8H (CGT→CAT)	grxA ←	glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	891,153	0	C	T	100.0%	123.9 / NA	38	R8H (CGT→CAT)	grxA	glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/19);  total (19/19)

ATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATG  >  W3110S.gb/891098‑891208                                                        |                                                        aTTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGAcc                                                     >  1:675644/1‑60 (MQ=255) aTTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCa                                                    >  1:1239539/1‑61 (MQ=255) aTTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCa                                                    >  1:2059365/1‑61 (MQ=255)          tttCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACg                                           <  1:1388186/61‑1 (MQ=255)          tttCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACg                                           <  1:3907906/61‑1 (MQ=255)                       tCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACgg                                          <  1:1230532/49‑1 (MQ=255)                       tCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACgg                                          <  1:4347627/49‑1 (MQ=255)                       tCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACgg                                          <  1:1911916/49‑1 (MQ=255)                       tCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACgg                                          <  1:3642394/49‑1 (MQ=255)                       tCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACgg                                          <  1:4199403/49‑1 (MQ=255)                       tCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACgg                                          <  1:3995358/49‑1 (MQ=255)                       tCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACgg                                          <  1:3800254/49‑1 (MQ=255)                       tCTTTTGCACGCACACAGTAAGGGCAACCCGAATGACCAAAAATAACgg                                          <  1:3787298/49‑1 (MQ=255)                               acgcacACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTctcctc                      <  1:2975094/61‑1 (MQ=255)                               acgcacACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTctcctc                      <  1:900593/61‑1 (MQ=255)                               acgcacACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTctcctc                      <  1:4294148/61‑1 (MQ=255)                               acgcacACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTctcctc                      <  1:563099/61‑1 (MQ=255)                               acgcacACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTctcctc                      <  1:1920359/61‑1 (MQ=255)                               acgcacACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTctcctc                      <  1:2764102/61‑1 (MQ=255)                               acgcacACAGTAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTctcctc                      <  1:214654/61‑1 (MQ=255)                                    cacaGTAAGGGCAACCCGAATGACCAAAAATAACgg                                          <  1:4255828/36‑1 (MQ=255)                                         tAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGAtt               >  1:1212109/1‑58 (MQ=255)                                         tAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGAtt               >  1:2860729/1‑58 (MQ=255)                                         tAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGAtt               >  1:1501777/1‑58 (MQ=255)                                         tAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGAtt               >  1:1635002/1‑58 (MQ=255)                                         tAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGAtt               >  1:171441/1‑58 (MQ=255)                                         tAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGAtt               >  1:4105835/1‑58 (MQ=255)                                         tAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGAtt               >  1:2573954/1‑58 (MQ=255)                                         tAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGAtt               >  1:3363936/1‑58 (MQ=255)                                         tAAGGGCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGAtt               >  1:3265240/1‑58 (MQ=255)                                             ggCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCt        >  1:3942547/1‑61 (MQ=255)                                             ggCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCt        >  1:2546597/1‑61 (MQ=255)                                             ggCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCt        >  1:3518530/1‑61 (MQ=255)                                             ggCAACCCGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCt        >  1:813174/1‑61 (MQ=255)                                                  cccGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATg  >  1:2042120/1‑62 (MQ=255)                                                  cccGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATg  >  1:2810158/1‑62 (MQ=255)                                                  cccGAATGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATg  >  1:3321036/1‑62 (MQ=255)                                                    cGAATGACCAAAAATAACGGTTTGCATTATTTCTctcctc                      <  1:736879/40‑1 (MQ=255)                                                        |                                                        ATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATG  >  W3110S.gb/891098‑891208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑