| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 922,613 | G→A | L615L (CTG→CTA) | macB → | fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 922,613 | 0 | G | A | 100.0% | 14.5 / NA | 6 | L615L (CTG→CTA) | macB | fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/6); total (0/6) | |||||||||||
TGGGAGATTGGTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACC > W3110S.gb/922581‑922663 | tGGGAGATTGGTTTTTCACCGTTGGCGCTGCTACTGGCGTTTCTCTGCTCGACGGTCACCgg < 1:1985482/62‑1 (MQ=255)tGGGAGATTGGTTTTTCACCGTTGGCGCTGCTACTGGCGTTTCTCTGCTCGACGGTCACCgg < 1:3987379/62‑1 (MQ=255)tGGGAGATTGGTTTTTCACCGTTGGCGCTGCTACTGGCGTTTCTCTGCTCGACGGTCACCgg < 1:454343/62‑1 (MQ=255) ttGGCGCTGCTACTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTAcc < 1:140806/62‑1 (MQ=255) ttGGCGCTGCTACTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTAcc < 1:3389420/62‑1 (MQ=255) ttGGCGCTGCTACTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTAcc < 1:454059/62‑1 (MQ=255) gctactgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTAcc < 1:3207907/54‑1 (MQ=255) gctactgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTAcc < 1:3665267/54‑1 (MQ=255) | TGGGAGATTGGTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACC > W3110S.gb/922581‑922663 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |