Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,007,501	A→C	N174K (AAT→AAG)	ycbX ←	predicted 2Fe‑2S cluster‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,007,501	0	A	C	100.0%	214.5 / NA	57	N174K (AAT→AAG)	ycbX	predicted 2Fe‑2S cluster‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (42/15);  total (42/15)

CAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGGAG  >  W3110S.gb/1007443‑1007551                                                           |                                                   cAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTg                                                 <  1:2970770/62‑1 (MQ=255) cAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTg                                                 <  1:4168029/62‑1 (MQ=255) cAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTg                                                 <  1:2286204/62‑1 (MQ=255)    tGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGCCGCGCCGGAAACCACCAGCTTgggg                                              >  1:649506/1‑62 (MQ=255)    tGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTgggg                                              >  1:1128917/1‑62 (MQ=255)    tGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTgggg                                              >  1:1349740/1‑62 (MQ=255)    tGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTgggg                                              >  1:612351/1‑62 (MQ=255)    tGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTgggg                                              >  1:2284531/1‑62 (MQ=255)      cGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCtt                                                  >  1:1548138/1‑56 (MQ=255)      cGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGc                                             >  1:4654860/1‑61 (MQ=255)      cGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGc                                             >  1:4089518/1‑61 (MQ=255)      cGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGc                                             >  1:54247/1‑61 (MQ=255)           cACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAAtt                                       >  1:2976915/1‑62 (MQ=255)             cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc                                     >  1:164998/1‑62 (MQ=255)             cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc                                     >  1:1187273/1‑62 (MQ=255)             cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc                                     >  1:2823108/1‑62 (MQ=255)             cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc                                     >  1:2693470/1‑62 (MQ=255)             cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc                                     >  1:2663425/1‑62 (MQ=255)             cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc                                     >  1:635642/1‑62 (MQ=255)             cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc                                     >  1:2558737/1‑62 (MQ=255)             cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc                                     >  1:24627/1‑62 (MQ=255)             cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc                                     >  1:1648458/1‑62 (MQ=255)             cTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGc                                     >  1:543615/1‑62 (MQ=255)              tttCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCt                                    <  1:412404/62‑1 (MQ=255)              tttCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCt                                    <  1:1453175/62‑1 (MQ=255)              tttCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCt                                    <  1:4529978/62‑1 (MQ=255)              tttCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCt                                    <  1:1597931/62‑1 (MQ=255)              tttCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCt                                    <  1:344323/62‑1 (MQ=255)               ttCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCt                                    <  1:3450828/61‑1 (MQ=255)               ttCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCt                                    <  1:3445242/61‑1 (MQ=255)                  cAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTcc                                  <  1:4113409/60‑1 (MQ=255)                  cAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTcc                                  <  1:970182/60‑1 (MQ=255)                  cAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCa                                 <  1:3438152/61‑1 (MQ=255)                  cAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCa                                 <  1:877994/61‑1 (MQ=255)                  cAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAt                                >  1:2803324/1‑62 (MQ=255)                  cAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAt                                >  1:1049242/1‑62 (MQ=255)                   aGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCa                                 <  1:3122343/60‑1 (MQ=255)                                ccAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCg                  >  1:3815709/1‑62 (MQ=255)                                ccAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCg                  >  1:2530233/1‑62 (MQ=255)                                ccAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCg                  >  1:2476553/1‑62 (MQ=255)                                ccAGGCTGACGCGCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCg                  >  1:2388185/1‑62 (MQ=255)                                         cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt                             >  1:3481359/1‑42 (MQ=255)                                         cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt                             >  1:3708982/1‑42 (MQ=255)                                         cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt                             >  1:945758/1‑42 (MQ=255)                                         cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt                             >  1:1095966/1‑42 (MQ=255)                                         cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt                             >  1:1404854/1‑42 (MQ=255)                                         cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt                             >  1:2059053/1‑42 (MQ=255)                                         cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt                             >  1:236528/1‑42 (MQ=255)                                         cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt                             >  1:4630305/1‑42 (MQ=255)                                         cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt                             >  1:4257729/1‑42 (MQ=255)                                         cgcgCCGGAAACCACCAGCTTGGGGCGGAATTGCTCCAtttt                             >  1:2646487/1‑42 (MQ=255)                                                 aaaCCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGgag  >  1:4545534/1‑61 (MQ=255)                                                 aaaCCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGgag  >  1:1978547/1‑61 (MQ=255)                                                 aaaCCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGgag  >  1:1571188/1‑61 (MQ=255)                                                 aaaCCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGgag  >  1:4397146/1‑61 (MQ=255)                                                 aaaCCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGgag  >  1:3496105/1‑61 (MQ=255)                                                 aaaCCACCAGCTTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGgag  >  1:2467151/1‑61 (MQ=255)                                                           |                                                   CAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACCACCAGATTGGGGCGGAATTGCTCCATTTTTACACTGGCCGGACAACGTTGTTGGAG  >  W3110S.gb/1007443‑1007551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑