Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,008,014	T→A	T3T (ACA→ACT)	ycbX ←	predicted 2Fe‑2S cluster‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,008,014	0	T	A	100.0%	52.2 / NA	17	T3T (ACA→ACT)	ycbX	predicted 2Fe‑2S cluster‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (8/9);  total (8/9)

GCATGTGTAAGACCAATGCCGCGCATCGATTTAACAGGATGAATAAAAAGCCGGATTAATGTCGCCACGGTGCGGTCCTCAAATGAAAATAAGCCCTCAACTTTATGACATG  >  W3110S.gb/1007955‑1008066                                                            |                                                     gCATGTGTAAGACCAATGCCGCGCATCGATTTAACAGGATGAATAAAAAGCCGGATTAAAGt                                                    >  1:606977/1‑62 (MQ=255) gCATGTGTAAGACCAATGCCGCGCATCGATTTAACAGGATGAATAAAAAGCCGGATTAAAGt                                                    >  1:452993/1‑62 (MQ=255)                    cgcgCATCGATTTAACAGGATGAATAAAAAGCCGGATTAAAGTCGCCACGGTGCGGTCCTCa                                 >  1:1587465/1‑62 (MQ=255)                    cgcgCATCGATTTAACAGGATGAATAAAAAGCCGGATTAAAGTCGCCACGGTGCGGTCCTCa                                 >  1:1496789/1‑62 (MQ=255)                                      gATGAATAAAAAGCCGGATTAAAGTCGCCACGGTGCGGTCCTCAAATGaa                           >  1:1672581/1‑50 (MQ=255)                                      gATGAATAAAAAGCCGGATTAAAGTCGCCACGGTGCGGTCCTCAAATGaa                           >  1:453074/1‑50 (MQ=255)                                      gATGAATAAAAAGCCGGATTAAAGTCGCCACGGTGCGGTCCTCAAATGaa                           >  1:382595/1‑50 (MQ=255)                                      gATGAATAAAAAGCCGGATTAAAGTCGCCACGGTGCGGTCCTCAAATGAAAATAAGCCCTCa               >  1:1651729/1‑62 (MQ=255)                                       aTGAATAAAAAGCCGGATTAAAGTCGCCACGGTGCGGTCCTCAAATGAAAATAAGCCCTCaa              <  1:3825707/62‑1 (MQ=255)                                       aTGAATAAAAAGCCGGATTAAAGTCGCCACGGTGCGGTCCTCAAATGAAAATAAGCCCTCaa              <  1:4374481/62‑1 (MQ=255)                                       aTGAATAAAAAGCCGGATTAAAGTCGCCACGGTGCGGTCCTCAAATGAAAATAAGCCCTCaa              <  1:3277109/62‑1 (MQ=255)                                       aTGAATAAAAAGCCGGATTAAAGTCGCCACGGTGCGGTCCTCAAATGAAAATAAGCCCTCa               <  1:2253153/61‑1 (MQ=255)                                        tGAATAAAAAGCCGGATTAAAGTCGCCACGGTGCGGTCCTCAAATGAAAATAAGCCCTCaa              <  1:787790/61‑1 (MQ=255)                                                 aGCCGGATTAAAGTCGCCACGGTGCGGTCCTCAAATGAAAATAAGCCCTCa               <  1:4403056/51‑1 (MQ=255)                                                 aGCCGGATTAAAGTCGCCACGGTGCGGTCCTCAAATGAAAATAAGCCCTCa               <  1:4497532/51‑1 (MQ=255)                                                         tAAAGTCGCCACGGTGCGGTCCTCAAATGAAAATAAGCCCTCAACTTTatgacatg  <  1:1994885/56‑1 (MQ=255)                                                         tAAAGTCGCCACGGTGCGGTCCTCAAATGAAAATAAGCCCTCAACTTTatgacatg  <  1:1760691/56‑1 (MQ=255)                                                            |                                                     GCATGTGTAAGACCAATGCCGCGCATCGATTTAACAGGATGAATAAAAAGCCGGATTAATGTCGCCACGGTGCGGTCCTCAAATGAAAATAAGCCCTCAACTTTATGACATG  >  W3110S.gb/1007955‑1008066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑