Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	257,873	A→G	K15K (AAA→AAG)	crl →	DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	257,873	0	A	G	100.0%	181.3 / NA	49	K15K (AAA→AAG)	crl	DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/4);  total (45/4)

GGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTT  >  W3110S.gb/257819‑257931                                                       |                                                           ggAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCg                                                     >  1:3961988/1‑62 (MQ=255) ggAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCg                                                     >  1:2724588/1‑62 (MQ=255) ggAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCg                                                     >  1:1607794/1‑62 (MQ=255) ggAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCg                                                     >  1:788550/1‑62 (MQ=255) ggAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCg                                                     >  1:4667302/1‑62 (MQ=255) ggAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCg                                                     >  1:591302/1‑62 (MQ=255)  gagaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTAcc                                                      >  1:2169535/1‑60 (MQ=255)  gagaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGc                                                    >  1:1195379/1‑62 (MQ=255)  gagaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGc                                                    >  1:3205400/1‑62 (MQ=255)  gagaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGc                                                    >  1:4543301/1‑62 (MQ=255)  gagaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGc                                                    >  1:2368201/1‑62 (MQ=255)  gagaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGc                                                    >  1:725104/1‑62 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGtt                                                          >  1:2361398/1‑44 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGtt                                                          >  1:1402248/1‑44 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGtt                                                          >  1:666314/1‑44 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGtt                                                          >  1:3065258/1‑44 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGtt                                                          >  1:2385861/1‑44 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGtt                                                          >  1:3843801/1‑44 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGtt                                                          >  1:1880651/1‑44 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGtt                                                          >  1:2159217/1‑44 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGtt                                                          >  1:2136309/1‑44 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGtt                                                          >  1:4671641/1‑44 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGta                                        >  1:3081944/1‑62 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGta                                        >  1:2568538/1‑62 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGta                                        >  1:3032298/1‑62 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGta                                        >  1:3020400/1‑62 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGta                                        >  1:3871489/1‑62 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGta                                        >  1:3304796/1‑62 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGta                                        >  1:720993/1‑62 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGta                                        >  1:2242762/1‑62 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGta                                        >  1:1994556/1‑62 (MQ=255)              aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGt                                         >  1:2919901/1‑61 (MQ=255)              aCGTTACCGAGTGGACACACGAAGAGCAGATTGATCAAAAAGtt                                                          >  1:3249084/1‑44 (MQ=38)                      gAGTTGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGt                                 <  1:824204/61‑1 (MQ=255)                                  gAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTg                      >  1:836301/1‑60 (MQ=255)                                  gAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTg                      >  1:4410161/1‑60 (MQ=255)                                  gAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTg                      >  1:927735/1‑60 (MQ=255)                                  gAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTg                      >  1:1482813/1‑60 (MQ=255)                                  gAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTg                      >  1:2532143/1‑60 (MQ=255)                                      agCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCaaa                  >  1:1423593/1‑60 (MQ=255)                                      agCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAg                 >  1:358502/1‑61 (MQ=255)                                      agCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAg                 >  1:363852/1‑61 (MQ=255)                                      agCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAg                 >  1:3775000/1‑61 (MQ=255)                                      agCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAg                 >  1:4683543/1‑61 (MQ=255)                                               aTCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGAtt       >  1:3382312/1‑62 (MQ=255)                                               aTCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGAtt       >  1:1662126/1‑62 (MQ=255)                                                tCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGAtt       <  1:139820/61‑1 (MQ=255)                                                tCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGAtt       <  1:624666/61‑1 (MQ=255)                                                tCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGAtt       <  1:1985202/61‑1 (MQ=255)                                                   aaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt   >  1:3971333/1‑62 (MQ=255)                                                   aaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt   >  1:3337468/1‑62 (MQ=255)                                                   aaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt   >  1:3065854/1‑62 (MQ=255)                                                   aaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt   >  1:3003444/1‑62 (MQ=255)                                                   aaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt   >  1:1762446/1‑62 (MQ=255)                                                   aaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt   >  1:1969089/1‑62 (MQ=255)                                                   aaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt   >  1:4397397/1‑62 (MQ=255)                                                   aaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt   >  1:2252161/1‑62 (MQ=255)                                                   aaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt   >  1:4571480/1‑62 (MQ=255)                                                   aaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt   >  1:4260353/1‑62 (MQ=255)                                                   aaaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtt    >  1:1866754/1‑61 (MQ=255)                                                    aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAg                        >  1:855339/1‑40 (MQ=255)                                                    aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:4020847/1‑62 (MQ=255)                                                    aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:4120379/1‑62 (MQ=255)                                                    aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:2927075/1‑62 (MQ=255)                                                    aaagTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:2485626/1‑62 (MQ=255)                                                    aaagTTTAACGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCtttt  >  1:2019632/1‑62 (MQ=255)                                                       |                                                           GGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTT  >  W3110S.gb/257819‑257931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑