Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,008,134	C→T	intergenic (‑112/‑132)	ycbX ← / → ycbY	predicted 2Fe‑2S cluster‑containing protein/predicted methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,008,134	0	C	T	100.0%	265.5 / NA	79	intergenic (‑112/‑132)	ycbX/ycbY	predicted 2Fe‑2S cluster‑containing protein/predicted methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/42);  total (37/42)

TTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTT  >  W3110S.gb/1008076‑1008189                                                           |                                                        ttaGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGt                                                      >  1:3816352/1‑62 (MQ=255) ttaGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGt                                                      >  1:594621/1‑62 (MQ=255)  taGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGt                                                      <  1:4022590/61‑1 (MQ=255)  taGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGt                                                      <  1:3135230/61‑1 (MQ=255)  taGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGt                                                      <  1:3118646/61‑1 (MQ=255)  taGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGt                                                      <  1:336158/61‑1 (MQ=255)  taGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGt                                                      <  1:3655344/61‑1 (MQ=255)  taGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGt                                                      <  1:4366363/61‑1 (MQ=255)  taGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGt                                                      <  1:1723355/61‑1 (MQ=255)  taGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGt                                                      <  1:1482977/61‑1 (MQ=255)  taGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGt                                                      <  1:503929/61‑1 (MQ=255)  taGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGt                                                      <  1:988484/61‑1 (MQ=255)  taGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGt                                                      <  1:727174/61‑1 (MQ=255)      tataATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGc                                                 <  1:1405054/62‑1 (MQ=255)      tataATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGc                                                 <  1:1131311/62‑1 (MQ=255)      tataATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGc                                                 <  1:1138800/62‑1 (MQ=255)          aTGCGCAACAATTTTCTTGGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTg                                             >  1:4588550/1‑62 (MQ=255)          aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGtt                                                     >  1:629460/1‑54 (MQ=255)          aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGc                                                 <  1:3110788/58‑1 (MQ=255)          aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGc                                                 <  1:2984366/58‑1 (MQ=255)          aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGc                                                 <  1:1879606/58‑1 (MQ=255)          aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGc                                                 <  1:3349408/58‑1 (MQ=255)          aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGc                                                 <  1:454426/58‑1 (MQ=255)          aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGc                                                 <  1:3359714/58‑1 (MQ=255)          aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTg                                             >  1:3215149/1‑62 (MQ=255)          aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTg                                             >  1:413176/1‑62 (MQ=255)          aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTg                                             >  1:2936633/1‑62 (MQ=255)          aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTg                                             >  1:3220351/1‑62 (MQ=255)          aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTg                                             >  1:2799917/1‑62 (MQ=255)          aTGCGCAACAATTTTCTTAGAGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTg                                             >  1:2143958/1‑62 (MQ=255)                 acaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGt                                          >  1:4669662/1‑58 (MQ=255)                 acaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGt                                          >  1:2607443/1‑58 (MQ=255)                 acaaTTTACTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATg                                      >  1:2096430/1‑62 (MQ=255)                      tttCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGc                                                 <  1:713070/46‑1 (MQ=255)                                 cTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGt                      <  1:2682630/62‑1 (MQ=255)                                 cTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGt                      <  1:3968523/62‑1 (MQ=255)                                 cTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGt                      <  1:1212202/62‑1 (MQ=255)                                 cTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGt                      <  1:4375101/62‑1 (MQ=255)                                 cTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGt                      <  1:750111/62‑1 (MQ=255)                                  tGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGt                      <  1:394876/61‑1 (MQ=255)                                   gTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTAc                    <  1:2053206/62‑1 (MQ=255)                                             gCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACg           <  1:1692062/61‑1 (MQ=255)                                               tattttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACgata        >  1:1015599/1‑59 (MQ=255)                                               tattttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTc        >  1:1036108/1‑62 (MQ=255)                                               tattttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTc        >  1:1546524/1‑62 (MQ=255)                                               tattttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTc        >  1:4391718/1‑62 (MQ=255)                                               tattttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTc        >  1:1298795/1‑62 (MQ=255)                                               tattttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTc        >  1:691715/1‑62 (MQ=255)                                                attttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCAc                        <  1:1007318/45‑1 (MQ=255)                                                attttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCAc                        <  1:4611695/45‑1 (MQ=255)                                                attttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCAc                        <  1:4467512/45‑1 (MQ=255)                                                attttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCAc                        <  1:2993526/45‑1 (MQ=255)                                                attttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCAc                        <  1:2457402/45‑1 (MQ=255)                                                attttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCAc                        <  1:2485839/45‑1 (MQ=255)                                                attttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCAc                        <  1:2591995/45‑1 (MQ=255)                                                  tttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCgg     >  1:1819223/1‑62 (MQ=255)                                                  tttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCgg     >  1:3465911/1‑62 (MQ=255)                                                  tttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCgg     >  1:3464497/1‑62 (MQ=255)                                                  tttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCgg     >  1:1474072/1‑62 (MQ=255)                                                   ttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATc                             <  1:3706172/37‑1 (MQ=255)                                                   ttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATc                             <  1:1297548/37‑1 (MQ=255)                                                   ttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATc                             <  1:1783049/37‑1 (MQ=255)                                                   ttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATc                             <  1:629336/37‑1 (MQ=255)                                                   ttatttGTTAGTTTGGCCCTGGGTTATGCTCGAAATc                             <  1:2527437/37‑1 (MQ=255)                                                      tttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGttt  <  1:1314874/61‑1 (MQ=255)                                                       ttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtg                 >  1:1193451/1‑45 (MQ=255)                                                       ttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtg                 >  1:533178/1‑45 (MQ=255)                                                       ttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtg                 >  1:1385386/1‑45 (MQ=255)                                                       ttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtg                 >  1:1716547/1‑45 (MQ=255)                                                       ttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtg                 >  1:1647890/1‑45 (MQ=255)                                                       ttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtg                 >  1:4383544/1‑45 (MQ=255)                                                       ttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtg                 >  1:1731005/1‑45 (MQ=255)                                                       ttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtg                 >  1:2284213/1‑45 (MQ=255)                                                       ttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtg                 >  1:2403900/1‑45 (MQ=255)                                                       ttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtg                 >  1:3676442/1‑45 (MQ=255)                                                       ttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtg                 >  1:3511764/1‑45 (MQ=255)                                                       ttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtg                 >  1:2709137/1‑45 (MQ=255)                                                       ttGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtg                 >  1:2895655/1‑45 (MQ=255)                                                       ttGTTAGTTTGGACCTGGGTTATGCTCGAAATCCTCACGTACTtg                 >  1:4175557/1‑45 (MQ=25)                                                           |                                                        TTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTT  >  W3110S.gb/1008076‑1008189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑