Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,032,415	C→T	intergenic (‑281/‑146)	serT ← / → hyaA	tRNA‑Ser/hydrogenase 1, small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,032,415	0	C	T	100.0%	244.5 / NA	74	intergenic (‑281/‑146)	serT/hyaA	tRNA‑Ser/hydrogenase 1, small subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/60);  total (14/60)

CACACAGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAA  >  W3110S.gb/1032358‑1032471                                                          |                                                         cacacaGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGc                                                      <  1:4334191/62‑1 (MQ=255) cacacaGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGc                                                      <  1:416424/62‑1 (MQ=255) cacacaGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGc                                                      <  1:2075577/62‑1 (MQ=255) cacacaGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGc                                                      <  1:53565/62‑1 (MQ=255)    acaGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGAc                                                   <  1:3085245/62‑1 (MQ=255)    acaGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGAc                                                   >  1:1219087/1‑62 (MQ=255)          tGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTgcgc                                             <  1:243484/62‑1 (MQ=255)          tGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTgcgc                                             <  1:237682/62‑1 (MQ=255)          tGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTgcgc                                             <  1:2260374/62‑1 (MQ=255)             aTTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTGCGCCa                                           <  1:4559321/61‑1 (MQ=255)              ttGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTGCGCCAGt                                         <  1:2620298/62‑1 (MQ=255)              ttGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTGCGCCAGt                                         <  1:3168096/62‑1 (MQ=255)                gTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTgc                                               >  1:1049366/1‑54 (MQ=255)                gTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTgc                                               >  1:4493498/1‑54 (MQ=255)                gTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTgc                                               >  1:3721308/1‑54 (MQ=255)                gTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTgc                                               >  1:3587713/1‑54 (MQ=255)                gTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTgc                                               >  1:323603/1‑54 (MQ=255)                gTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTgc                                               >  1:1779218/1‑54 (MQ=255)                gTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTgc                                               >  1:1202495/1‑54 (MQ=255)                gTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTgc                                               >  1:4646733/1‑54 (MQ=255)                 ttttGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTGCGCCAGTGc                                       >  1:367584/1‑61 (MQ=255)                 ttttGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTGCGCCAGTGc                                       >  1:3154232/1‑61 (MQ=255)                 ttttGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTGCGCCAGTGc                                       >  1:2803488/1‑61 (MQ=255)                   ttGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTGCGCCAGTGCAg                                     <  1:4531911/61‑1 (MQ=255)                            aGTTTCACTACGCTTTATTAACAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGc                           <  1:2758389/62‑1 (MQ=255)                                  aCTACGCTTTATTAACAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCg                      <  1:1591397/61‑1 (MQ=255)                                  aCTACGCTTTATTAACAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCg                      <  1:399577/61‑1 (MQ=255)                                  aCTACGCTTTATTAACAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCg                      <  1:1748712/61‑1 (MQ=255)                                   cTACGCTTTATTAACAATACTTTTTGGCGACGTTCGCCAGTGCAGAAGGATGAGCTTTCGtt                    >  1:294841/1‑62 (MQ=255)                                   cTACGCTTTATTAACAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGtt                    >  1:1719278/1‑62 (MQ=255)                                   cTACGCTTTATTAACAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGt                     <  1:1164800/61‑1 (MQ=255)                                             ttaACAATTCTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCAtc           <  1:4529039/61‑1 (MQ=255)                                             ttaACAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCAtc           <  1:3753084/61‑1 (MQ=255)                                             ttaACAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCAtc           <  1:2677257/61‑1 (MQ=255)                                             ttaACAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCAtc           <  1:3481746/61‑1 (MQ=255)                                             ttaACAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCAtc           <  1:2933348/61‑1 (MQ=255)                                             ttaACAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCAtc           <  1:201447/61‑1 (MQ=255)                                             ttaACAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCAtc           <  1:1429705/61‑1 (MQ=255)                                              taACAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCAtct          <  1:681788/61‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCATAAGGATGAGCttt                        <  1:2477556/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:1737608/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:150998/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:4378378/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:700535/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:4029688/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:1301894/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:3989565/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:3807701/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:2494642/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:1730389/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:1203260/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:3543952/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:2290625/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:175240/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:2824757/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:2962648/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:3082840/45‑1 (MQ=255)                                                aCAATACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCttt                        <  1:2971254/45‑1 (MQ=255)                                                    tACTTTTTGGCGACGTTCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:1355398/48‑1 (MQ=255)                                                    tACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:780009/48‑1 (MQ=255)                                                    tACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:4649006/48‑1 (MQ=255)                                                    tACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:607768/48‑1 (MQ=255)                                                    tACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:4326601/48‑1 (MQ=255)                                                    tACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:431218/48‑1 (MQ=255)                                                    tACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:3264424/48‑1 (MQ=255)                                                    tACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:3039194/48‑1 (MQ=255)                                                    tACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:3001932/48‑1 (MQ=255)                                                    tACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:2494599/48‑1 (MQ=255)                                                    tACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:2034123/48‑1 (MQ=255)                                                    tACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:1600366/48‑1 (MQ=255)                                                    tACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:1429921/48‑1 (MQ=255)                                                    tACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:1324960/48‑1 (MQ=255)                                                    tACTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTc                 <  1:111680/48‑1 (MQ=255)                                                     aCTTTTTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGaa  <  1:1098146/62‑1 (MQ=255)                                                          |                                                         CACACAGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAA  >  W3110S.gb/1032358‑1032471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑