Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,036,407	A→G	E72E (GAA→GAG)	hyaD →	protein involved in processing of HyaA and HyaB proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,036,407	0	A	G	100.0%	24.1 / NA	8	E72E (GAA→GAG)	hyaD	protein involved in processing of HyaA and HyaB proteins
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/1);  total (7/1)

CGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGC  >  W3110S.gb/1036362‑1036430                                              |                        cgcCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGctggagcctg                     >  1:1450368/1‑50 (MQ=255) cgcCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAGCCTGGAACGCTGCGaa         >  1:1842808/1‑62 (MQ=255) cgcCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAGCCTGGAACGCTGCGaa         >  1:2011243/1‑62 (MQ=255) cgcCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAGCCTGGAACGCTGCGaa         >  1:3227949/1‑62 (MQ=255) cgcCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAGCCTGGAACGCTGCGaa         >  1:718115/1‑62 (MQ=255) cgcCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAGCCTGGAACGCTGCGaa         >  1:846367/1‑62 (MQ=255) cgcCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAGCCTGGAACGCTGCGaa         >  1:868933/1‑62 (MQ=255) cgcCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAGCCTGGAACGCTGCGa          >  1:2899960/1‑61 (MQ=255)         aTCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAGCCTGGAACGCTGCGAACCTATGc  <  1:2888315/61‑1 (MQ=255)            tGTTGATTCTCGATGCCATTGACTACGGGctggagcctggaac                 <  1:3010507/43‑1 (MQ=255)            tGTTGATTCTCGATGCCATTGACTACGGGctggagcctggaac                 <  1:3841734/43‑1 (MQ=255)                                              |                        CGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGC  >  W3110S.gb/1036362‑1036430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑