Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,074,318	G→T	A39E (GCG→GAG)	ycdM ←	predicted monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,074,318	0	G	T	100.0%	88.6 / NA	28	A39E (GCG→GAG)	ycdM	predicted monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/8);  total (20/8)

CTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCA  >  W3110S.gb/1074263‑1074368                                                        |                                                   cTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTggg                                              >  1:488196/1‑62 (MQ=255) cTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTggg                                              >  1:451201/1‑62 (MQ=255) cTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTggg                                              >  1:4265694/1‑62 (MQ=255) cTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTggg                                              >  1:3448848/1‑62 (MQ=255) cTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTggg                                              >  1:2627711/1‑62 (MQ=255)           tGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGa                                     <  1:93923/61‑1 (MQ=255)           tGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGa                                     <  1:617329/61‑1 (MQ=255)                aaTGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATTagccagc                               <  1:780450/62‑1 (MQ=255)                aaTGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGagccagc                               <  1:3520178/62‑1 (MQ=255)                aaTGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGagccagc                               <  1:1233811/62‑1 (MQ=255)                           tCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGcc                     >  1:3312948/1‑61 (MQ=255)                           tCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGcc                     >  1:3383659/1‑61 (MQ=255)                           tCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGcc                     >  1:1207759/1‑61 (MQ=255)                           tCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGcc                     >  1:3845563/1‑61 (MQ=255)                           tCAGTTCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGcc                     >  1:718033/1‑61 (MQ=255)                               ttCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAg                <  1:1163148/62‑1 (MQ=255)                               ttCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAg                <  1:257675/62‑1 (MQ=255)                               ttCAAAGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAg                <  1:4554152/62‑1 (MQ=255)                                   aaGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTa             >  1:883664/1‑61 (MQ=255)                                   aaGGTCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTa             >  1:2168856/1‑61 (MQ=255)                                       tCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAAt        >  1:24633/1‑62 (MQ=255)                                       tCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAAt        >  1:4338562/1‑62 (MQ=255)                                       tCGGCATGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAAt        >  1:3230384/1‑62 (MQ=255)                                             tGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCgtt                           >  1:2574602/1‑37 (MQ=255)                                             tGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCa  >  1:4586847/1‑62 (MQ=255)                                             tGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCa  >  1:702926/1‑62 (MQ=255)                                             tGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCa  >  1:246726/1‑62 (MQ=255)                                             tGTACTGCGGCTCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCa  >  1:1130229/1‑62 (MQ=255)                                                        |                                                   CTCCGCTTTTTGCACAATGGCTTTATTCAGTTCAAAGGTCGGCATGTACTGCGGCGCGTGGGTCGAAATGAGCCAGCCGTTGTTGCCAATAGGTACGAATACGCCA  >  W3110S.gb/1074263‑1074368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑