Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,084,947	A→G	K384E (AAA→GAA)	ycdB →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,084,947	0	A	G	100.0%	78.5 / NA	22	K384E (AAA→GAA)	ycdB	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/18);  total (4/18)

GTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTT  >  W3110S.gb/1084902‑1085001                                              |                                                       gTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGa                                        >  1:4548798/1‑62 (MQ=255)           aaCACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTgga                               >  1:1446359/1‑61 (MQ=255)           aaCACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTgga                               >  1:1557847/1‑61 (MQ=255)           aaCACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTgga                               >  1:2008522/1‑61 (MQ=255)                        aaaaGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc                 <  1:1210521/62‑1 (MQ=255)                        aaaaGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc                 <  1:3272552/62‑1 (MQ=255)                        aaaaGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc                 <  1:1127358/62‑1 (MQ=255)                        aaaaGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc                 <  1:1859636/62‑1 (MQ=255)                        aaaaGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc                 <  1:2827535/62‑1 (MQ=255)                         aaaGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc                 <  1:465951/61‑1 (MQ=255)                                   tGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcg        <  1:4344981/60‑1 (MQ=255)                                   tGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcg        <  1:3102668/60‑1 (MQ=255)                                   tGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcg        <  1:306819/60‑1 (MQ=255)                                   tGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcg        <  1:2340766/60‑1 (MQ=255)                                   tGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcg        <  1:1470124/60‑1 (MQ=255)                                   tGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATcggcg        <  1:1279829/60‑1 (MQ=255)                                       aGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGtt  <  1:468920/62‑1 (MQ=255)                                       aGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGtt  <  1:840947/62‑1 (MQ=255)                                       aGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGtt  <  1:4318526/62‑1 (MQ=255)                                       aGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGtt  <  1:2524886/62‑1 (MQ=255)                                       aGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGtt  <  1:2008835/62‑1 (MQ=255)                                       aGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGtt  <  1:1826826/62‑1 (MQ=255)                                              |                                                       GTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTT  >  W3110S.gb/1084902‑1085001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑