Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,093,686	T→C	V130A (GTA→GCA)	ycdT →	predicted diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,093,686	0	T	C	100.0%	199.0 / NA	54	V130A (GTA→GCA)	ycdT	predicted diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/25);  total (29/25)

GCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAA  >  W3110S.gb/1093634‑1093741                                                     |                                                        gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTgg                                                    <  1:1254877/58‑1 (MQ=255) gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTgg                                                    <  1:4555620/58‑1 (MQ=255) gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTgg                                                    <  1:1637361/58‑1 (MQ=255) gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTgg                                                    <  1:3617458/58‑1 (MQ=255) gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTgg                                                    <  1:2545732/58‑1 (MQ=255) gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTgg                                                    <  1:2487116/58‑1 (MQ=255)  cAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTgg                                                    <  1:1097304/57‑1 (MQ=255)      cTATTTTTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTgg                                                    <  1:3527722/53‑1 (MQ=255)        attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                         >  1:2956815/1‑62 (MQ=255)        attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                         >  1:4492744/1‑62 (MQ=255)        attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                         >  1:4209478/1‑62 (MQ=255)        attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                         >  1:3760443/1‑62 (MQ=255)        attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                         >  1:3431912/1‑62 (MQ=255)        attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                         >  1:3097381/1‑62 (MQ=255)        attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                         >  1:2622320/1‑62 (MQ=255)        attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                         >  1:1665111/1‑62 (MQ=255)        attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                         >  1:1800456/1‑62 (MQ=255)        attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                         >  1:1803898/1‑62 (MQ=255)        attattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGt                                          >  1:2864886/1‑61 (MQ=255)              tGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                   <  1:816698/62‑1 (MQ=255)                       aGATGAGTTTTTGCATATTAATATTTCTGGCATTGGTGa                                                 <  1:503465/39‑1 (MQ=25)                           gAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                   <  1:2032099/49‑1 (MQ=255)                           gAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                   <  1:4058743/49‑1 (MQ=255)                           gAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                   <  1:504155/49‑1 (MQ=255)                           gAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                   <  1:3808622/49‑1 (MQ=255)                           gAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                   <  1:1070404/49‑1 (MQ=255)                           gAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgc                      <  1:2255521/62‑1 (MQ=255)                           gAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgc                      <  1:578438/62‑1 (MQ=255)                           gAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgc                      <  1:263036/62‑1 (MQ=255)                           gAGTTTGAGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgc                      <  1:3256047/62‑1 (MQ=255)                            aGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgc                      <  1:140139/61‑1 (MQ=255)                                  tgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                            >  1:3286402/1‑49 (MQ=255)                                  tgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                            >  1:1874408/1‑49 (MQ=255)                                  tgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                            >  1:1894566/1‑49 (MQ=255)                                  tgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                            >  1:1899966/1‑49 (MQ=255)                                  tgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                            >  1:4529297/1‑49 (MQ=255)                                  tgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                            >  1:2170676/1‑49 (MQ=255)                                  tgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                            >  1:4314217/1‑49 (MQ=255)                                  tgCATATCAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                   >  1:4690339/1‑42 (MQ=38)                                               ttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAACCAGCGCAATTTATTTTCCaaaaaa  >  1:3844319/1‑62 (MQ=255)                                               ttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgc                      >  1:2264014/1‑42 (MQ=255)                                               ttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa  >  1:890638/1‑62 (MQ=255)                                               ttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa  >  1:1609629/1‑62 (MQ=255)                                               ttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa  >  1:4030186/1‑62 (MQ=255)                                               ttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa  >  1:3838788/1‑62 (MQ=255)                                               ttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa  >  1:1830677/1‑62 (MQ=255)                                               ttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa  >  1:1924693/1‑62 (MQ=255)                                               ttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa  >  1:201138/1‑62 (MQ=255)                                               ttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaa  >  1:2127652/1‑62 (MQ=255)                                                tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgcg                     <  1:4287194/42‑1 (MQ=255)                                                tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgcg                     <  1:392586/42‑1 (MQ=255)                                                tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgcg                     <  1:4553811/42‑1 (MQ=255)                                                tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgcg                     <  1:2944583/42‑1 (MQ=255)                                                tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgcg                     <  1:2408274/42‑1 (MQ=255)                                                     |                                                        GCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAA  >  W3110S.gb/1093634‑1093741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑