Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,097,861	G→A	noncoding (33/88 nt)	serX ←	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,097,861	0	G	A	100.0%	72.6 / NA	28	noncoding (33/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/9);  total (19/9)

TGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGT  >  W3110S.gb/1097841‑1097909                     |                                                 tGCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg         <  1:980081/62‑1 (MQ=18) tGCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg         <  1:1600373/62‑1 (MQ=18) tGCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg         <  1:797942/62‑1 (MQ=18) tGCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg         <  1:2141082/62‑1 (MQ=18) tGCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg         <  1:2321909/62‑1 (MQ=18) tGCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg         <  1:4466623/62‑1 (MQ=18) tGCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg         <  1:4392628/62‑1 (MQ=18) tGCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg         >  1:3967657/1‑62 (MQ=18) tGCCGTATACACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg         >  1:3857074/1‑62 (MQ=18)        taCACACTTTCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCg         <  1:2141801/55‑1 (MQ=16)           acacTTTCCAAGCGTGCTCCTTCAGCGACTCGGACACCTCACCAAATTGTCg         <  1:2639439/52‑1 (MQ=11)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:3547444/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:635463/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:558633/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:503380/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:3882075/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:3847787/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:3709071/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:1010079/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:3391469/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:3379130/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:3240391/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:2902547/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:2762062/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:1763605/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:1692269/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGt  >  1:1513145/1‑55 (MQ=255)               tttCCAAGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCAGt  >  1:4132065/1‑55 (MQ=14)                     |                                                 TGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGT  >  W3110S.gb/1097841‑1097909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑