Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,099,547	C→T	A143V (GCC→GTC)	ycdX →	predicted zinc‑binding hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,099,547	0	C	T	100.0%	114.3 / NA	35	A143V (GCC→GTC)	ycdX	predicted zinc‑binding hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/11);  total (24/11)

TATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCA  >  W3110S.gb/1099500‑1099602                                                |                                                        tataATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAagca                                            <  1:1537349/61‑1 (MQ=255) tataATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAagca                                            <  1:4418582/61‑1 (MQ=255) tataATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAagca                                            <  1:566578/61‑1 (MQ=255)              cccGGAAATCCCAAATATGAAATAGATGTGAAAGTCg                                                       >  1:572048/1‑37 (MQ=255)              cccGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTGAAGAAGCCGCGAAACATCa                              >  1:4591983/1‑62 (MQ=255)              cccGGAAATCCCAAATATGAAATAGATGTGAAAGTCGTTGCTCAagcagc                                          >  1:2547478/1‑50 (MQ=255)                          aaaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGa                  <  1:1752519/62‑1 (MQ=255)                          aaaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGa                  <  1:4503751/62‑1 (MQ=255)                          aaaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGa                  <  1:487207/62‑1 (MQ=255)                          aaaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGa                  <  1:3147116/62‑1 (MQ=255)                          aaaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGa                  <  1:3756644/62‑1 (MQ=255)                           aaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGaa                 >  1:3912630/1‑62 (MQ=255)                           aaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGaa                 >  1:462316/1‑62 (MQ=255)                           aaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGaa                 >  1:447926/1‑62 (MQ=255)                           aaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGaa                 >  1:4250119/1‑62 (MQ=255)                           aaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGaa                 >  1:1050224/1‑62 (MQ=255)                           aaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGaa                 >  1:412435/1‑62 (MQ=255)                           aaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGaa                 >  1:2706598/1‑62 (MQ=255)                           aaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGaa                 >  1:2618251/1‑62 (MQ=255)                           aaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGaa                 >  1:2390342/1‑62 (MQ=255)                           aaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGaa                 >  1:650628/1‑62 (MQ=255)                           aaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGaa                 >  1:852981/1‑62 (MQ=255)                           aaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGa                  >  1:4184338/1‑61 (MQ=255)                           aaTATGAAATAGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGa                  >  1:4244632/1‑61 (MQ=255)                                     aGATGTGAAAGTCTTTGCTGAAGAAGCCGCGAAACATCAg                             >  1:4234931/1‑40 (MQ=25)                                     aGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTg                          >  1:4021405/1‑43 (MQ=255)                                     aGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAAtt       >  1:4161975/1‑62 (MQ=255)                                     aGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAAtt       >  1:696245/1‑62 (MQ=255)                                     aGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAAtt       >  1:4353855/1‑62 (MQ=255)                                     aGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAAtt       >  1:1422632/1‑62 (MQ=255)                                     aGATGTGAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAAtt       >  1:1096498/1‑62 (MQ=255)                                        tgtgAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTcc     <  1:3454008/61‑1 (MQ=255)                                        tgtgAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTcc     <  1:805446/61‑1 (MQ=255)                                          tgAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCa  >  1:2875817/1‑62 (MQ=255)                                          tgAAAGTCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCa  <  1:198731/62‑1 (MQ=255)                                                |                                                        TATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGATGTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCCTCA  >  W3110S.gb/1099500‑1099602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑