Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	267,502	G→T	P228H (CCT→CAT)	ykfA ←	predicted GTP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	267,502	0	G	T	100.0%	151.7 / NA	46	P228H (CCT→CAT)	ykfA	predicted GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (33/13);  total (33/13)

AGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTA  >  W3110S.gb/267468‑267552                                   |                                                   aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa                                              <  1:1029777/41‑1 (MQ=255) aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa                                              <  1:79488/41‑1 (MQ=255) aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa                                              <  1:3707303/41‑1 (MQ=255) aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa                                              <  1:3875419/41‑1 (MQ=255) aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa                                              <  1:1675320/41‑1 (MQ=255) aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa                                              <  1:1890241/41‑1 (MQ=255) aGCCTGTACCCGGACTACCGTCGTGCGAAAGGAATGATGGa                                              <  1:3658979/41‑1 (MQ=255)  gCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa                                              <  1:1499861/40‑1 (MQ=255)   ccTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa                                              <  1:3479199/39‑1 (MQ=255)        tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa                                       >  1:4499157/1‑41 (MQ=255)        tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa                                       >  1:4313752/1‑41 (MQ=255)        tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa                                       >  1:4219198/1‑41 (MQ=255)        tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa                                       >  1:4157882/1‑41 (MQ=255)        tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa                                       >  1:1613506/1‑41 (MQ=255)        tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa                                       >  1:3849360/1‑41 (MQ=255)        tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa                                       >  1:3769786/1‑41 (MQ=255)        tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa                                       >  1:2499066/1‑41 (MQ=255)        tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa                                       >  1:1426819/1‑41 (MQ=255)        tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa                                       >  1:2781907/1‑41 (MQ=255)        tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa                                       >  1:705014/1‑41 (MQ=255)            ggACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACg               <  1:1049612/61‑1 (MQ=255)            ggACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACg               <  1:2380460/61‑1 (MQ=255)            ggACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACg               <  1:1701395/61‑1 (MQ=255)                  ccGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACg               <  1:1220318/55‑1 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:416105/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:701422/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:4638133/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:4303307/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:4246625/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:2188676/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:79992/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:3955873/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:3945614/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:2195548/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:2243006/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:3814025/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:277886/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:2797648/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg        >  1:3470561/1‑55 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcag         >  1:2177501/1‑54 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcag         >  1:4647883/1‑54 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACtt   >  1:946051/1‑60 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACtt   >  1:1145826/1‑60 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTa  >  1:4400520/1‑61 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTa  >  1:2189894/1‑61 (MQ=255)                         gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTa  >  1:3128626/1‑61 (MQ=255)                                   |                                                   AGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTA  >  W3110S.gb/267468‑267552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑