Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,115,786	T→C	intergenic (‑28/+55)	msyB ← / ← mdtG	hypothetical protein/predicted drug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,115,786	0	T	C	100.0%	206.9 / NA	56	intergenic (‑28/+55)	msyB/mdtG	hypothetical protein/predicted drug efflux system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/34);  total (22/34)

TTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCA  >  W3110S.gb/1115732‑1115843                                                       |                                                          ttcttcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAgg                                                     <  1:2778676/61‑1 (MQ=255) ttcttcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAgg                                                     <  1:720126/61‑1 (MQ=255) ttcttcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAgg                                                     <  1:2263400/61‑1 (MQ=255) ttcttcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAgg                                                     <  1:321804/61‑1 (MQ=255) ttcttcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAgg                                                     <  1:616674/61‑1 (MQ=255) ttcttcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAgg                                                     <  1:3690746/61‑1 (MQ=255) ttcttcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAgg                                                     <  1:4290150/61‑1 (MQ=255) ttcttcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAgg                                                     <  1:3904322/61‑1 (MQ=255) ttcttcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAgg                                                     <  1:1788425/61‑1 (MQ=255) ttcttcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAgg                                                     <  1:1778901/61‑1 (MQ=255)      cAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAg                                               >  1:4219307/1‑62 (MQ=255)      cAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAg                                               >  1:3796588/1‑62 (MQ=255)      cAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAg                                               >  1:560162/1‑62 (MQ=255)      cAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAg                                               >  1:681838/1‑62 (MQ=255)      cAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAg                                               >  1:683519/1‑62 (MQ=255)                          atcaCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCg                               >  1:4156699/1‑58 (MQ=255)                          atcaCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCg                               >  1:955160/1‑58 (MQ=255)                          atcaCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCg                               >  1:4658058/1‑58 (MQ=255)                          atcaCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCg                               >  1:754537/1‑58 (MQ=255)                          atcaCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCg                               >  1:252201/1‑58 (MQ=255)                          atcaCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCg                               >  1:1144884/1‑58 (MQ=255)                          atcaCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCg                               >  1:1950587/1‑58 (MQ=255)                          atcaCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCttt                           >  1:2430082/1‑62 (MQ=255)                          atcaCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCttt                           >  1:4572773/1‑62 (MQ=255)                          atcaCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCttt                           >  1:4549766/1‑62 (MQ=255)                          atcaCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCttt                           >  1:1633649/1‑62 (MQ=255)                          atcaCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCttt                           >  1:4287865/1‑62 (MQ=255)                          atcaCGATTCTCACTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCg                               >  1:3143553/1‑58 (MQ=255)                                   ctcCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTa                   >  1:606862/1‑61 (MQ=255)                                   ctcCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTa                   >  1:413109/1‑61 (MQ=255)                                     cccTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTg                                       <  1:2578981/39‑1 (MQ=255)                                     cccTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTg                                       <  1:1136057/39‑1 (MQ=255)                                     cccTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTg                                       <  1:1856144/39‑1 (MQ=255)                                     cccTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTg                                       <  1:2844289/39‑1 (MQ=255)                                        tttGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAg             <  1:845808/62‑1 (MQ=255)                                        tttGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAg             <  1:1745854/62‑1 (MQ=255)                                        tttGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAg             <  1:1508881/62‑1 (MQ=255)                                        tttGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAg             <  1:4553406/62‑1 (MQ=255)                                        tttGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAg             <  1:2173065/62‑1 (MQ=255)                                        tttGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAg             <  1:4622714/62‑1 (MQ=255)                                        tttGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAg             <  1:1626648/62‑1 (MQ=255)                                        tttGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAg             <  1:504405/62‑1 (MQ=255)                                             gTTGATGAGGCTTCAGGGAAAAGGATATCTGATTCTCCGCTTTTGAAAGTATGAAAGGCGa         >  1:1035039/1‑61 (MQ=255)                                             gTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGa         >  1:4710183/1‑61 (MQ=255)                                                   gaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCa  <  1:4707545/62‑1 (MQ=255)                                                   gaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCa  <  1:2625665/62‑1 (MQ=255)                                                   gaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCa  <  1:1432067/62‑1 (MQ=255)                                                   gaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCa  <  1:1465034/62‑1 (MQ=255)                                                   gaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCa  <  1:1513470/62‑1 (MQ=255)                                                   gaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCa  <  1:3786805/62‑1 (MQ=255)                                                   gaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCa  <  1:1762964/62‑1 (MQ=255)                                                   gaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCa  <  1:1871374/62‑1 (MQ=255)                                                   gaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCa  <  1:4080585/62‑1 (MQ=255)                                                    aGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCa  <  1:3636494/61‑1 (MQ=255)                                                    aGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCa  <  1:3726066/61‑1 (MQ=255)                                                    aGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCa  <  1:2709389/61‑1 (MQ=255)                                                       |                                                          TTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCA  >  W3110S.gb/1115732‑1115843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑