Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,142,123	A→G	I172V (ATA→GTA)	flgL →	flagellar hook‑filament junction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,142,123	0	A	G	100.0%	51.4 / NA	15	I172V (ATA→GTA)	flgL	flagellar hook‑filament junction protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/13);  total (2/13)

AAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCACACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAA  >  W3110S.gb/1142083‑1142179                                         |                                                         aaGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACaaaa                                     >  1:2247134/1‑62 (MQ=255) aaGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACaaaa                                     >  1:3501529/1‑62 (MQ=255)    tATTAACCAACAGGTCGATGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACAAAAttt                                  <  1:4411905/62‑1 (MQ=255)    tATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACAAAAttt                                  <  1:1590530/62‑1 (MQ=255)    tATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACAAAAttt                                  <  1:1950690/62‑1 (MQ=255)    tATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACAAAAttt                                  <  1:2874480/62‑1 (MQ=255)    tATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACAAAAttt                                  <  1:3561669/62‑1 (MQ=255)    tATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACAAAAttt                                  <  1:376898/62‑1 (MQ=255)    tATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACAAAAttt                                  <  1:4038283/62‑1 (MQ=255)    tATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACAAAAttt                                  <  1:4072527/62‑1 (MQ=255)    tATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACAAAAttt                                  <  1:4206480/62‑1 (MQ=255)     aTTAAACTACAGGTCGATGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACAAAAttt                                  <  1:1322864/61‑1 (MQ=255)          acaacaGTTCGATGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACAAAATTTTCGAc                             <  1:2642351/61‑1 (MQ=255)          acaacaGGTCGATGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACAAAATTTTCGAc                             <  1:479892/61‑1 (MQ=255)                      tGCTTCGCGTTCGATGGTGGTAGGGCACACGGGTGACAAAAttt                                  <  1:2104439/44‑1 (MQ=255)                                    tggtggTAGGGCACACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGaa  <  1:3797114/62‑1 (MQ=255)                                         |                                                         AAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCACACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAA  >  W3110S.gb/1142083‑1142179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑