Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,210,070	C→T	S121F (TCC→TTC)	ymfS →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,210,070	0	C	T	100.0%	38.3 / NA	14	S121F (TCC→TTC)	ymfS	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/14);  total (0/14)

AATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAG  >  W3110S.gb/1210019‑1210113                                                    |                                            aaTATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATTCAGTGCGCCa                                   <  1:1829950/62‑1 (MQ=255)  atatAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATTCAGTGCGCCa                                   <  1:4291056/61‑1 (MQ=255)              gCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATTCAGTGCGCCAGATATTGAATGGc                      <  1:4389661/62‑1 (MQ=255)              gCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATTCAGTGCGCCAGATATTGAATGGc                      <  1:614145/62‑1 (MQ=255)                                  tGGAACTGGTTGATACATTCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAg  <  1:2520013/62‑1 (MQ=255)                                  tGGAACTGGTTGATACATTCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAg  <  1:4547372/62‑1 (MQ=255)                                  tGGAACTGGTTGATACATTCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAg  <  1:890259/62‑1 (MQ=255)                                   ggAACTGGTTGATACATTTAGTGCGCCAGATATTg                            <  1:2051309/35‑1 (MQ=21)                                   ggAACTGGTTGATACATTCAGTGCGCCAGATATTg                            <  1:1585930/35‑1 (MQ=255)                                   ggAACTGGTTGATACATTCAGTGCGCCAGATATTg                            <  1:2119950/35‑1 (MQ=255)                                   ggAACTGGTTGATACATTCAGTGCGCCAGATATTg                            <  1:2507505/35‑1 (MQ=255)                                   ggAACTGGTTGATACATTCAGTGCGCCAGATATTg                            <  1:2765910/35‑1 (MQ=255)                                   ggAACTGGTTGATACATTCAGTGCGCCAGATATTg                            <  1:334612/35‑1 (MQ=255)                                   ggAACTGGTTGATACATTCAGTGCGCCAGATATTg                            <  1:789259/35‑1 (MQ=255)                                                    |                                            AATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAG  >  W3110S.gb/1210019‑1210113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑