Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,224,919	G→A	intergenic (‑423/‑123)	ymgG ← / → ymgH	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,224,919	0	G	A	100.0%	83.8 / NA	26	intergenic (‑423/‑123)	ymgG/ymgH	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/22);  total (4/22)

ATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTAC  >  W3110S.gb/1224873‑1224962                                               |                                            aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat                               <  1:913916/61‑1 (MQ=255) aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat                               <  1:853109/61‑1 (MQ=255) aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat                               <  1:1744085/61‑1 (MQ=255) aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat                               <  1:4424908/61‑1 (MQ=255) aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat                               <  1:4240871/61‑1 (MQ=255) aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat                               <  1:1973363/61‑1 (MQ=255) aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat                               <  1:3950069/61‑1 (MQ=255) aTTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat                               <  1:1288464/61‑1 (MQ=255)  ttAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtattat                               <  1:3230000/60‑1 (MQ=255)  ttAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGcattat                               <  1:1741502/60‑1 (MQ=21)       cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa                        <  1:3619646/62‑1 (MQ=255)       cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa                        <  1:716857/62‑1 (MQ=255)       cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa                        <  1:4487621/62‑1 (MQ=255)       cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa                        <  1:4223623/62‑1 (MQ=255)       cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa                        <  1:3885945/62‑1 (MQ=255)       cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa                        <  1:2492335/62‑1 (MQ=255)       cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa                        <  1:188261/62‑1 (MQ=255)       cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa                        <  1:186721/62‑1 (MQ=255)       cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa                        <  1:1668393/62‑1 (MQ=255)       cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa                        <  1:1645533/62‑1 (MQ=255)       cATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa                        <  1:1624981/62‑1 (MQ=255)        attttattttGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGaaaa                        <  1:1728450/61‑1 (MQ=255)           ttattttGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGt                    >  1:741566/1‑62 (MQ=255)                                        gATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTac  >  1:4142484/1‑51 (MQ=255)                                        gATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTac  >  1:2045729/1‑51 (MQ=255)                                        gATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTac  >  1:4403186/1‑51 (MQ=255)                                               |                                            ATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTAC  >  W3110S.gb/1224873‑1224962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑