Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,254,608	T→C	intergenic (+46/+54)	dhaR → / ← ycgV	predicted DNA‑binding transcriptional regulator, dihydroxyacetone/predicted adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,254,608	0	T	C	100.0%	43.7 / NA	13	intergenic (+46/+54)	dhaR/ycgV	predicted DNA‑binding transcriptional regulator, dihydroxyacetone/predicted adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/2);  total (11/2)

CAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCT  >  W3110S.gb/1254567‑1254652                                          |                                             cAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAAtt                           >  1:1025480/1‑61 (MQ=255) cAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAAtt                           >  1:1447098/1‑61 (MQ=255) cAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAAtt                           >  1:152521/1‑61 (MQ=255) cAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAAtt                           >  1:43177/1‑61 (MQ=255) cAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAAtt                           >  1:4649/1‑61 (MQ=255) cAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAAtt                           >  1:950299/1‑61 (MQ=255)    aaaCGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTc                        >  1:1303683/1‑61 (MQ=255)    aaaCGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTc                        >  1:2066652/1‑61 (MQ=255)    aaaCGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTc                        >  1:2230246/1‑61 (MQ=255)    aaaCGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTc                        >  1:2581385/1‑61 (MQ=255)    aaaCGATTTCTGATACATCAGAGTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTc                        >  1:503875/1‑61 (MQ=255)                         agTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCt  <  1:1801265/62‑1 (MQ=255)                         agTGATCTGTATTTCATCCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCt  <  1:983779/62‑1 (MQ=255)                                          |                                             CAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCT  >  W3110S.gb/1254567‑1254652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑