Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,255,918	C→T	G538S (GGC→AGC)	ycgV ←	predicted adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,255,918	0	C	T	100.0%	221.8 / NA	66	G538S (GGC→AGC)	ycgV	predicted adhesin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/26);  total (40/26)

CTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCT  >  W3110S.gb/1255863‑1255967                                                        |                                                  ctactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTaacaac                                             >  1:2841487/1‑62 (MQ=255) ctactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTaacaac                                             >  1:4485285/1‑62 (MQ=255) ctactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTaacaac                                             >  1:2261604/1‑62 (MQ=255) ctactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTaacaac                                             >  1:4527760/1‑62 (MQ=255) ctactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTaacaac                                             >  1:2768562/1‑62 (MQ=255) ctactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTaacaac                                             >  1:799056/1‑62 (MQ=255) ctactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTaacaac                                             >  1:1802425/1‑62 (MQ=255) ctactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTaacaac                                             >  1:86226/1‑62 (MQ=255) ctactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTaacaac                                             >  1:1749274/1‑62 (MQ=255) ctactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTaacaac                                             >  1:925728/1‑62 (MQ=255)  tactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAacaaca                                            <  1:351360/62‑1 (MQ=255)  tactCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAacaaca                                            <  1:4370074/62‑1 (MQ=255)   actCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAacaaca                                            <  1:4641722/61‑1 (MQ=255)      cccGCTGATATTCAATAAATCCCCTTTATTATTGACGCCATTACCCTCGCTAACAACATCa                                         <  1:3586984/61‑1 (MQ=255)      cccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCa                                         <  1:3062675/61‑1 (MQ=255)      cccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCa                                         <  1:2891431/61‑1 (MQ=255)      cccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCa                                         <  1:1057377/61‑1 (MQ=255)       ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAg                                        >  1:2415915/1‑61 (MQ=255)       ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAg                                        >  1:3962216/1‑61 (MQ=255)       ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGc                                       >  1:4388753/1‑62 (MQ=255)       ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGc                                       >  1:4508397/1‑62 (MQ=255)       ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGc                                       >  1:522134/1‑62 (MQ=255)       ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGc                                       >  1:759050/1‑62 (MQ=255)       ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGc                                       >  1:876552/1‑62 (MQ=255)       ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGc                                       >  1:1348177/1‑62 (MQ=255)       ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGc                                       >  1:1773778/1‑62 (MQ=255)       ccGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGc                                       >  1:2721163/1‑62 (MQ=255)           tGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACg                                    >  1:2183287/1‑61 (MQ=255)           tGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACg                                    >  1:274762/1‑61 (MQ=255)           tGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACg                                    >  1:3072040/1‑61 (MQ=255)           tGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACg                                    >  1:521620/1‑61 (MQ=255)           tGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACg                                    >  1:2361878/1‑61 (MQ=255)           tGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACg                                    >  1:2389177/1‑61 (MQ=255)           tGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACg                                    >  1:3338651/1‑61 (MQ=255)           tGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACg                                    >  1:339420/1‑61 (MQ=255)           tGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACg                                    >  1:2067952/1‑61 (MQ=255)           tGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACg                                    >  1:3547152/1‑61 (MQ=255)           tGATATTCAATAAATCCCCTTTATTACTAACGCCATTACCCTCGCTAACAACATCAGCACg                                    >  1:2637163/1‑61 (MQ=255)                       aaTCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTa                       <  1:2170619/62‑1 (MQ=255)                       aaTCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTa                       <  1:2835300/62‑1 (MQ=255)                       aaTCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTa                       <  1:4166480/62‑1 (MQ=255)                       aaTCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTa                       <  1:2283851/62‑1 (MQ=255)                       aaTCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTa                       <  1:4578127/62‑1 (MQ=255)                       aaTCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTa                       <  1:3664896/62‑1 (MQ=255)                       aaTCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTa                       <  1:1805251/62‑1 (MQ=255)                       aaTCCCCTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTa                       <  1:2994361/62‑1 (MQ=255)                            ccTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGtt                  >  1:665111/1‑62 (MQ=255)                            ccTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGtt                  >  1:3635491/1‑62 (MQ=255)                            ccTTTATTATTAACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGtt                  >  1:32386/1‑62 (MQ=255)                                 attattaACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCg              >  1:3895193/1‑61 (MQ=255)                                 attattaACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCg              >  1:312613/1‑61 (MQ=255)                                 attattaACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCg              >  1:455101/1‑61 (MQ=255)                                  ttattaACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGc             <  1:3812432/61‑1 (MQ=255)                                  ttattaACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGc             <  1:4147496/61‑1 (MQ=255)                                  ttattaACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGc             <  1:1470668/61‑1 (MQ=255)                                  ttattaACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGc             <  1:2236322/61‑1 (MQ=255)                                   tattaACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGc             <  1:2977769/60‑1 (MQ=255)                                    attaACGCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCa          <  1:3808465/62‑1 (MQ=255)                                          gCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtct    <  1:2218386/62‑1 (MQ=255)                                          gCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtct    <  1:2618487/62‑1 (MQ=255)                                          gCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtct    <  1:3520143/62‑1 (MQ=255)                                          gCCATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtct    <  1:348318/62‑1 (MQ=255)                                           ccATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtctc   >  1:3109919/1‑62 (MQ=255)                                            cATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTa                       <  1:3843230/41‑1 (MQ=255)                                            cATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTctct  >  1:3131986/1‑62 (MQ=255)                                            cATTACCCTCGCTAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTctct  >  1:1664433/1‑62 (MQ=255)                                                        |                                                  CTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCT  >  W3110S.gb/1255863‑1255967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑