| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,268,022 | A→G | T118A (ACG→GCG) | prmC → | N5‑glutamine methyltransferase, modifies release factors RF‑1 and RF‑2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,268,022 | 0 | A | G | 95.8% | 77.6 / ‑3.8 | 24 | T118A (ACG→GCG) | prmC | N5‑glutamine methyltransferase, modifies release factors RF‑1 and RF‑2 |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (15/8); total (16/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
TGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATT > W3110S.gb/1267963‑1268078 | tggtggAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGGCg > 1:3712917/1‑62 (MQ=255) tGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGTGGCGATTGCGCTTGCGCTg < 1:886728/61‑1 (MQ=255) tGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGATTGCGCTTGCGCTg < 1:1532516/61‑1 (MQ=255) tGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGATTGCGCTTGCGCTg < 1:2018905/61‑1 (MQ=255) tGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGATTGCGCTTGCGCTg < 1:2454253/61‑1 (MQ=255) tGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGATTGCGCTTGCGCTg < 1:313884/61‑1 (MQ=255) tGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGATTGCGCTTGCGCTg < 1:3307156/61‑1 (MQ=255) tGAACAACCTTGCCGTATTCTCGATCTCGGGGCGGGTACCGGGGCGATTGCGCTTGCGCTg < 1:3909391/61‑1 (MQ=255) gATCTCGGGGCGGGGACCGGGGCGATTGCGCTTGCGCTg < 1:2920656/39‑1 (MQ=37) tctcGGGGCGGGTACCGGGGCGATtgcgcttgcgct > 1:3465117/1‑36 (MQ=255) tctcGGGGCGGGTACCGGGGCGATtgcgcttgcgct > 1:4474819/1‑36 (MQ=255) tctcGGGGCGGGTACCGGGGCGATtgcgcttgcgct > 1:4242648/1‑36 (MQ=255) tctcGGGGCGGGTACCGGGGCGATtgcgcttgcgct > 1:1210051/1‑36 (MQ=255) tctcGGGGCGGGTACCGGGGCGATtgcgcttgcgct > 1:3324937/1‑36 (MQ=255) tctcGGGGCGGGTACCGGGGCGATtgcgcttgcgct > 1:271334/1‑36 (MQ=255) tctcGGGGCGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGaa > 1:2879466/1‑61 (MQ=255) tctcGGGGCGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGaa > 1:4641473/1‑61 (MQ=255) tcGGGGCGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAAtt > 1:3542/1‑62 (MQ=255) tcGGGGCGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAAtt > 1:334499/1‑62 (MQ=255) tcGGGGCGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAAtt > 1:4060494/1‑62 (MQ=255) tcGGGGCGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAAtt > 1:4254018/1‑62 (MQ=255) tcGGGGCGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAAtt > 1:794203/1‑62 (MQ=255) tcGGGGCGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAAtt > 1:966556/1‑62 (MQ=255) tcGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAAtt > 1:3055479/1‑62 (MQ=255) | TGGTGGAGCAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATT > W3110S.gb/1267963‑1268078 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |